EIF3K antibody (C-Term)

Catalog No. ABIN952051

Product Details anti-EIF3K Antibody

Target: EIF3K (Eukaryotic Translation Initiation Factor 3 Subunit K (EIF3K))

Binding Specificity: AA 175-204, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3K antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to EIF3K.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 175-204 amino acids from the C-terminal region of human EIF3K

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-EIF3K antibody (ABIN952051)

López-González, Schlüter, Aso, Garcia-Esparcia, Ansoleaga, LLorens, Carmona, Moreno, Fuso, Portero-Otin, Pamplona, Pujol, Ferrer: "Neuroinflammatory signals in Alzheimer disease and APP/PS1 transgenic mice: correlations with plaques, tangles, and oligomeric species." in: Journal of neuropathology and experimental neurology, Vol. 74, Issue 4, pp. 319-44, (2015) (PubMed).

Zhou, Sandercock, Fraser, Ridlova, Stephens, Schenauer, Yokoi-Fong, Barsky, Leary, Hershey, Doudna, Robinson: "Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18139-44, (2008) (PubMed).

Lin, Chen, Lin, Wang, Inoko, Inagaki, Wu, Chen: "eIF3k regulates apoptosis in epithelial cells by releasing caspase 3 from keratin-containing inclusions." in: Journal of cell science, Vol. 121, Issue Pt 14, pp. 2382-93, (2008) (PubMed).

Damoc, Fraser, Zhou, Videler, Mayeur, Hershey, Doudna, Robinson, Leary: "Structural characterization of the human eukaryotic initiation factor 3 protein complex by mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 7, pp. 1135-46, (2007) (PubMed).

Masutani, Sonenberg, Yokoyama, Imataka: "Reconstitution reveals the functional core of mammalian eIF3." in: The EMBO journal, Vol. 26, Issue 14, pp. 3373-83, (2007) (PubMed).

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Schweizer, Bowden, Coulombe, Langbein, Lane, Magin, Maltais, Omary, Parry, Rogers, Wright: "New consensus nomenclature for mammalian keratins." in: The Journal of cell biology, Vol. 174, Issue 2, pp. 169-74, (2006) (PubMed).

Target Details for EIF3K

Target: EIF3K (Eukaryotic Translation Initiation Factor 3 Subunit K (EIF3K))

Alternative Name: EIF3K / EIF3S12 (EIF3K Products)

Synonyms: eif3s12 antibody, EIF3S12 antibody, ACYPI000023 antibody, eIF3k antibody, EIF3-p28 antibody, HSPC029 antibody, M9 antibody, MSTP001 antibody, PLAC-24 antibody, PLAC24 antibody, PRO1474 antibody, PTD001 antibody, 1200009C21Rik antibody, Eif3s12 antibody, zgc:110726 antibody, eukaryotic translation initiation factor 3 subunit K L homeolog antibody, eukaryotic translation initiation factor 3 subunit K antibody, eukaryotic translation initiation factor 3, subunit K antibody, eukaryotic translation initiation factor 3 subunit k antibody, Eukaryotic translation initiation factor 3 subunit K antibody, eif3k.L antibody, EIF3K antibody, eif3k antibody, ANI_1_1610144 antibody, AOR_1_2266174 antibody, Tsp_00068 antibody, Tsp_13184 antibody, Eif3k antibody, LOC733122 antibody, eif-3.K antibody

Background: The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).Synonyms: Eukaryotic translation initiation factor 3 subunit 12, Eukaryotic translation initiation factor 3 subunit K, Muscle-specific gene M9 protein, PLAC-24, eIF-3 p25, eIF-3 p28

Molecular Weight: 25060 Da

Gene ID: 27335

NCBI Accession: NP_037366

Pathways: Ribonucleoprotein Complex Subunit Organization