NME1 antibody (Middle Region)

Catalog No. ABIN953624

The Rabbit Polyclonal anti-NME1 antibody has been validated for WB and EIA. It is suitable to detect NME1 in samples from Human and Mouse.

Quick Overview for NME1 antibody (Middle Region) (ABIN953624)

Target: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NME1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-NME1 Antibody

Binding Specificity: AA 28-57, Middle Region

Specificity: This antibody recognizes human and mouse NDP kinase A.

Purification: Purified through a Protein A column followed by peptide affinity purification

Immunogen: Synthetic peptide - KLH conjugated - corresponding to the central region (between 28-57aa) of human NDP kinase A.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NME1

Target: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Alternative Name: NDP Kinase A

Background: The NME1 gene was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.Synonyms: GAAD, Granzyme A-activated DNase, NDPKA, NM23, NME1, Nucleoside diphosphate kinase A, nm23-H1

Molecular Weight: 17149 Da

Gene ID: 4830

NCBI Accession: NP_000260

Pathways: Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process