L-Asparaginase (ANSZ) antibody

Details for Product No. ABIN95396
Antigen
Reactivity
Escherichia coli (E. coli)
6
1
1
Host
Rabbit
7
1
Clonality
Polyclonal
Conjugate
Un-conjugated
2
1
Application
ELISA
7
6
3
1
Options
Immunogen Asparaginase [E. coli]
Immunogentype:Native
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Antiserum
Antigen
Alternative Name L-Asparaginase
Background L-Asparaginase antibody detects bacterial Asparaginase. Asparaginase is an enzyme that catalyzes the hydrolysis of asparagine to aspartic acid. Asparaginases are naturally occurring enzymes expressed and produced by microorganisms. Colaspase is also known as L-asparaginase (E. coli). Anti-L-Asparaginase antibody is ideal for investigators invovled enzyme reseach.
Synonyms: L-asparaginase II
Gene ID 1039460
UniProt P00805
Application Notes Anti-L-Asparaginase antibody has been assayed against 1.0 ug of Asparaginase [E. coli] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:100,000 to 1:400,000 is suggested for this product.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 1 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
Image no. 1 for anti-L-Asparaginase (ANSZ) antibody (ABIN95396) Western Blot of Rabbit anti-L-Asparaginase Antibody. Lane 1: L-Asparaginase. Lane 2: ...
Product cited in: Charbonneau, Aubé, Rachel, Guerrero, Delorme, Breault-Turcot, Masson, Pelletier: "Development of Escherichia coli Asparaginase II for Immunosensing: A Trade-Off between Receptor Density and Sensing Efficiency." in: ACS omega, Vol. 2, Issue 5, pp. 2114-2125, 2018 (PubMed).