Factor VIII antibody
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- Target See all Factor VIII (F8) Antibodies
- Factor VIII (F8) (Coagulation Factor VIII (F8))
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Reactivity
- Human
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- This Factor VIII antibody is un-conjugated
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Application
- ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Gel Shift (GS)
- Brand
- IHC-plus™
- Specificity
- Recognizes human F. VIII as demonstrated by immunodiffusion and ELISA. No reactivity was observed with von Willebrand Factor.
- Purification
- Ammonium sulfate precipitation
- Immunogen
- Human Factor VIIIC (F. VIII) purified from F. VIII concentrate.
- Isotype
- IgG
- Top Product
- Discover our top product F8 Primary Antibody
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- Application Notes
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Approved: ELISA, GS, IHC, IHC-P (5 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 10 mM HEPES, pH 7.4, 0.15 M sodium chloride, 50 % glycerol.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- Factor VIII (F8) (Coagulation Factor VIII (F8))
- Alternative Name
- F8 / FVIII / Factor VIII (F8 Products)
- Synonyms
- fb61d02 antibody, wu:fb61d02 antibody, Cf-8 antibody, Cf8 antibody, FVIII antibody, AHF antibody, DXS1253E antibody, F8B antibody, F8C antibody, HEMA antibody, coagulation factor VIIi antibody, coagulation factor VIII antibody, coagulation factor VIII, procoagulant component antibody, f7i antibody, F8 antibody
- Background
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Name/Gene ID: F8
Synonyms: F8, AHF, Antihemophilic factor, Coagulation factor VIIIc, Coagulation factor VIII, DXS1253E, Factor VIII F8B, FVIII, HEMA, F8B, Factor VIII, Procoagulant component, F8C - Gene ID
- 2157
- UniProt
- P00451
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