LRP5 antibody (C-Term)

Catalog No. ABIN962131

Product Details anti-LRP5 Antibody

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LRP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: Recognizes human LRP5 protein. Western Blot tests using negative control mock pSEC-tag-transfected 293T cell lysates did not yield the target band. Species cross-reactivity: Mouse.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminal region of the human LRP5 protein. This intracellular region is identical to the corresponding mouse and rat LRP5 amino acid sequences.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF (2 - 3 μg/mL), WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunofluorescence and Western Blot. Immunofluorescence: 2-3 μg/mL, Note: For optimal results in immunofluorescence assays, cells should be fixed in 4 % paraformaldehyde/0.5 % Triton X-100/PBS for 10 minutes, permeabilized with 0.5 % Triton X-100/PBS for 5 minutes, and blocked with 5 % BSA/PBS for 30 minutes. Western Blot: 1-3 μg/mL, Single band at ~200kD. Positive control: Mouse LRP5-pSEC-tag-transfected 293T cells, MCF-7 human breast adenocarcinoma, T47D human breast ductal carcinoma, and NIH-3T3 mouse embryo fibroblast lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for LRP5

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Alternative Name: LRP5 (LRP5 Products)

Synonyms: Xlrp5 antibody, bmnd1 antibody, evr1 antibody, evr4 antibody, hbm antibody, lr3 antibody, lrp5-A antibody, lrp7 antibody, oppg antibody, ops antibody, opta1 antibody, vbch2 antibody, LRP5 antibody, LRP6 antibody, BMND1 antibody, EVR1 antibody, EVR4 antibody, HBM antibody, LR3 antibody, LRP7 antibody, OPPG antibody, OPS antibody, OPTA1 antibody, VBCH2 antibody, mKIAA4142 antibody, low-density lipoprotein receptor-related protein 5 antibody, LDL receptor related protein 5 antibody, LDL receptor related protein 5 L homeolog antibody, low density lipoprotein receptor-related protein 5 antibody, LDL receptor related protein 5 like antibody, LOC100147353 antibody, LRP5 antibody, lrp5.L antibody, lrp5 antibody, Lrp5 antibody, LRP5L antibody

Background: Name/Gene ID: LRP5

Synonyms: LRP5, EVR1, Exudative vitreoretinopathy 1, LRP7, OPTA1, OPPG, OPS, LR3, LRP-5, VBCH2, BMND1, EVR4

Gene ID: 4041

UniProt: O75197

Pathways: WNT Signaling, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation