V5 Epitope Tag antibody (Biotin)

Catalog No. ABIN962841

Product Details anti-V5 Epitope Tag Antibody (Biotin)

Target: V5 Epitope Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This V5 Epitope Tag antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Brand: THE™

Sequence: GKPIPNPLLG LDST

Specificity: THE^TM V5 Tag Antibody [Biotin], mAb, Mouse recognizes N-terminal and C-terminal V5 tagged fusion proteins.

Characteristics: The THE^TM V5 Tag Antibody [Biotin], mAb, Mouse is THE^TM V5 Tag Antibody conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.

Purification: Protein A affinity column

Immunogen: V5 tag peptide conjugated - KLH

Clone: 4C12E11

Isotype: IgG2a

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.005-0.05 µg/mL
Western blot: 0.2-1 µg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.

Buffer: PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Target Details for V5 Epitope Tag

Target: V5 Epitope Tag

Alternative Name: V5 Tag (V5 Epitope Tag Products)

Target Type: Tag

Background: V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).