CEBPD antibody (N-Term)

Catalog No. ABIN964606

Product Details anti-CEBPD Antibody

Target: CEBPD (CCAAT/enhancer Binding Protein (C/EBP), delta (CEBPD))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEBPD antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for mouse C/EBP delta protein. A BLAST analysis was used to suggest cross-reactivity with C/EBPd from human sources based on 100% homology with the immunizing sequence. No cross-reactivity is observed with mouse C/EBPa or C/EBPb. Reactivity with C/EBPd from other sources has not been determined.

Cross-Reactivity: Human

Characteristics: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression. Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T). Phosphorylation of C/EBPd may increase binding activity, whereas phosphorylation of the a and b family members may decrease binding affinity. C/EBPd is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.

Purification: purified

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse C/EBP.
Immunogen Type: RecombinantProtein

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 35 kDa in size corresponding to C/EBPd by western blotting in the appropriate cell lysate or extract.   The predicted molecular weight of mouse C/EBPd is 28.8 kDa. This antibody is capable of detecting both over-expressed and endogenous C/EBPd.

Comment:

Gene Name: CEBPD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.83 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is three (3) months from date of opening.

Expiry Date: 3 months

References for anti-CEBPD antibody (ABIN964606)

Puzio-Kuter, Laddha, Castillo-Martin, Sun, Cordon-Cardo, Chan, Levine: "Involvement of tumor suppressors PTEN and p53 in the formation of multiple subtypes of liposarcoma." in: Cell death and differentiation, Vol. 22, Issue 11, pp. 1785-91, (2015) (PubMed).

Sarkar, Sharan, Wang, Pawar, Cantwell, Johnson, Morrison, Wang, Sterneck: "Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPδ expression and contributes to transformation of breast tumor cells." in: Molecular and cellular biology, Vol. 32, Issue 2, pp. 320-32, (2012) (PubMed).

Balamurugan, Wang, Tsai, Sharan, Anver, Leighty, Sterneck: "The tumour suppressor C/EBPδ inhibits FBXW7 expression and promotes mammary tumour metastasis." in: The EMBO journal, Vol. 29, Issue 24, pp. 4106-17, (2011) (PubMed).

Target Details for CEBPD

Target: CEBPD (CCAAT/enhancer Binding Protein (C/EBP), delta (CEBPD))

Alternative Name: CEBP Delta (CEBPD Products)

Synonyms: C/EBP-delta antibody, CELF antibody, CRP3 antibody, NF-IL6-beta antibody, C/EBPd antibody, LOC398729 antibody, LOC398730 antibody, MGC114740 antibody, c/ebp-delta antibody, celf antibody, crp3 antibody, nf-il6-beta antibody, c/ebpd antibody, wu:fa99c04 antibody, wu:fb61h03 antibody, CEBPD antibody, cebpd antibody, CCAAT/enhancer binding protein (C/EBP), delta antibody, CCAAT/enhancer binding protein delta antibody, CCAAT/enhancer binding protein delta L homeolog antibody, CCAAT/enhancer binding protein delta S homeolog antibody, Cebpd antibody, CEBPD antibody, cebpd.L antibody, cebpd.S antibody, cebpd antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages.  Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression.  Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T).  Phosphorylation of C/EBPd may increase binding activity, whereas phosphorylation of the a and b family members may decrease binding affinity.  C/EBPd is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.
Synonyms: C/EBP related protein 3 antibody, CCAAT/enhancer binding protein delta antibody, CEBP D antibody, CEBPD antibody, CELF antibody, Crp 3 antibody, Crp3 antibody, NF IL6 beta antibody, Nuclear factor NF IL6 beta antibody

Gene ID: 12609, 110347410

UniProt: Q00322