TNF alpha antibody

Catalog No. ABIN964799

Product Details anti-TNF alpha Antibody

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This product was Protein-A purified from monospecific antiserum by chromatography. It is specific for swine TNFalpha protein. A BLAST analysis was used to suggest cross-reactivity with TNFalpha from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against TNFalpha from monkey, human, chimpanzee, macaque, baboon, vervet, mangabey, rough-toothed dolphin and beluga whale based on 94-95% homology, from manatee, dog, cat, panda, ferret, and bottle-nosed dolphin (92-93%), and horse, goat, bovine, sheep, rhinoceros, elk and nilgai based on 90-91% homology. Cross-reactivity with TNFalpha from sources other than swine has not been determined.

Characteristics: Tumor necrosis factor-alpha (TNFalpha) in humans is a pleiotropic inflammatory cytokine. It is expressed by many different stimulated cell types including monocytes, endothelial cells, fibroblasts and both T and B-lymphocytes, but especially by macrophages. It affects most organs. The production of TNF alpha is induced by IL-1, PDGF, IFN-beta, NGF, Oncostatin M and certain fungal, viral, and parasitic invasions. Bacterial lipopolysaccharide is an especially potent stimulus. The primary role of TNFalpha is in the regulation of immune cells. TNF alpha mediates septic shock in response to infection. It initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophages and neutrophils. TNFalpha secreted by macrophages causes blood clotting which helps contain infection. TNFalpha is also able to induce apoptotic cell death or inflammation, and to inhibit viral replication. It possesses growth stimulating, inhibitory, and self regulatory properties. Dysregulation of TNFalpha production has been implicated in a variety of human diseases, as well as cancer. The presence of TNFalpha is responsible for diverse immunomodulatory and toxic effects. For instance, TNFalpha induces neutrophil proliferation during inflammation, but it also induces neutrophil apoptosis upon binding to the TNF-R55 receptor. TNFalpha causes necrosis of some types of tumors, but promotes growth of other types. Low levels may aid in maintaining homeostasis by regulating the body's circadian rhythm, and may promote remodeling or replacement of injured and senescent tissue by stimulating fibroblast growth. High levels of TNFalpha correlate with increased risk of mortality.

Purification: purified

Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 154 amino acids of mature swine TNFalpha.
Immunogen Type: RecombinantProtein

Isotype: IgG

Application Details

Application Notes: This protein A purified antibody is suitable for ELISA and Western blotting.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.9 kDa in size corresponding to swine TNFα by western blotting in the appropriate cell lysate or extract.

Comment:

Gene Name: TNFA

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha (TNF alpha Products)

Synonyms: DIF antibody, TNF-alpha antibody, TNFA antibody, TNFSF2 antibody, RATTNF antibody, Tnfa antibody, tnf antibody, TNF-a antibody, TNFalpha antibody, Tnfsf1a antibody, TNFa antibody, cTNF antibody, Tnf-alpha antibody, tnfa-like antibody, TNF-ALPHA antibody, dif antibody, tnfa antibody, xtnf antibody, tnfsf2 antibody, tnf-alpha antibody, Cachectin antibody, tumor necrosis factor antibody, tumor necrosis factor b (TNF superfamily, member 2) antibody, tumor necrosis factor alpha antibody, tumor necrosis factor a (TNF superfamily, member 2) antibody, TNF antibody, Tnf antibody, tnf antibody, tnfb antibody, tnf-alpha antibody, LOC103694380 antibody, tnfa antibody

Background: Tumor necrosis factor-alpha (TNFα) in humans is a pleiotropic inflammatory cytokine. It is expressed by many different stimulated cell types including monocytes, endothelial cells, fibroblasts and both T and B-lymphocytes, but especially by macrophages. It affects most organs. The production of TNF alpha is induced by IL-1, PDGF, IFN-beta, NGF, Oncostatin M and certain fungal, viral, and parasitic invasions. Bacterial lipopolysaccharide is an especially potent stimulus. The primary role of TNFα is in the regulation of immune cells. TNF α mediates septic shock in response to infection. It initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophages and neutrophils. TNFα secreted by macrophages causes blood clotting which helps contain infection. TNFα is also able to induce apoptotic cell death or inflammation, and to inhibit viral replication. It possesses growth stimulating, inhibitory, and self regulatory properties. Dysregulation of TNFα production has been implicated in a variety of human diseases, as well as cancer. The presence of TNFα is responsible for diverse immunomodulatory and toxic effects. For instance, TNFα induces neutrophil proliferation during inflammation, but it also induces neutrophil apoptosis upon binding to the TNF-R55 receptor. TNFα causes necrosis of some types of tumors, but promotes growth of other types. Low levels may aid in maintaining homeostasis by regulating the body's circadian rhythm, and may promote remodeling or replacement of injured and senescent tissue by stimulating fibroblast growth. High levels of TNFα correlate with increased risk of mortality.
Synonyms: TNF-A, TNF-α, TNF alpha, cachexin , cachectin; tumor necrosis factor-alpha, TNFSF2, TNFSF1A.

Gene ID: 397086

NCBI Accession: NP_999187

UniProt: P23563

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome