CCL4 antibody

Catalog No. ABIN964801

Product Details anti-CCL4 Antibody

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for swine CCL4 protein. A BLAST analysis was used to suggest cross-reactivity with CCL4 from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against horse and panda CCL4 based on 98% homology, with bovine, dog, and rabbit based on 97% homology, macaque and feline based on 95%, with human and chimpanzee based on 94%, and with rat and opossum CCL4 based on 91% homology. Cross-reactivity with CCL4 from other sources has not been determined. The swine CCL4 sequence is also 86% homologous to swine CCL3L1.

Characteristics: In many species, both C-C chemokines macrophage inflammatory protein, or MIP-1 alpha (CCL3L1), and MIP-1 beta (CCL4) are structurally and functionally related CC chemokines. They are both potent chemoattractants for monocytes, which form an important component of the stroma of tumor tissue, and in humans may regulate tumor growth and associated inflammation. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. Both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes, but their effect on lymphocytes differ. MIP-1 alpha selectively attracts CD8+ lymphocytes, while MIP-1 beta selectively attracts CD4+ lymphocytes. They contain the four highly conserved cysteine residues present in CC chemokines. MIP-1 beta (CCL4) has specificity for CCR5 receptors. In humans, it is also a major HIV-suppressive factor produced by CD8+ T cells.

Purification: purified

Sterility: Sterile filtered

Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant protein raised in yeast, corresponding to the 69 amino acids of the mature swine CCL4/MIP1ß protein.
Immunogen Type: RecombinantProtein

Isotype: IgG

Application Details

Application Notes: This protein-A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7.8 kDa in size corresponding to swine CCL4 protein by western blotting in the appropriate cell lysate or extract.

Comment:

Gene Name: CCL4

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

Target Details for CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Alternative Name: MIP 1 beta (CCL4 Products)

Synonyms: ACT2 antibody, AT744.1 antibody, G-26 antibody, HC21 antibody, LAG-1 antibody, LAG1 antibody, MIP-1-beta antibody, MIP1B antibody, MIP1B1 antibody, SCYA2 antibody, SCYA4 antibody, CCL4 antibody, K203 antibody, Mip1-b antibody, Scya4 antibody, MIP-1beta antibody, MIP-1BETA antibody, MIP1b antibody, SCY4A antibody, Act-2 antibody, MIP-1B antibody, Mip1b antibody, ACT-2 antibody, AT744.2 antibody, CCL4L antibody, SCYA4L antibody, SCYA4L1 antibody, SCYA4L2 antibody, C-C motif chemokine ligand 4 antibody, chemokine (C-C motif) ligand 4 antibody, chemokine (C-C motif) ligand 4-like 1 antibody, C-C motif chemokine ligand 4 like 1 antibody, CCL4 antibody, Ccl4 antibody, CCL4L1 antibody

Background: In many species, both C-C chemokines macrophage inflammatory protein, or MIP-1 alpha (CCL3L1), and MIP-1 beta (CCL4) are structurally and functionally related CC chemokines. They are both potent chemoattractants for monocytes, which form an important component of the stroma of tumor tissue, and in humans may regulate tumor growth and associated inflammation. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. Both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes, but their effect on lymphocytes differ. MIP-1 alpha selectively attracts CD8+ lymphocytes, while MIP-1 beta selectively attracts CD4+ lymphocytes. They contain the four highly conserved cysteine residues present in CC chemokines. MIP-1 beta (CCL4) has specificity for CCR5 receptors. In humans, it is also a major HIV-suppressive factor produced by CD8+ T cells.
Synonyms: CCL4, C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, ACT-2, MIP-1ß

Gene ID: 396668

UniProt: Q711P4