Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (HRP)

Details for Product No. ABIN964977, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
6229
2030
1776
1135
782
648
372
305
34
34
31
15
12
12
12
12
11
7
3
3
1
1
1
1
1
1
1
Reactivity
Rabbit
3078
2913
2380
1662
1509
785
737
489
476
410
401
395
341
298
271
160
159
111
54
34
30
26
16
15
12
10
8
7
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Host
Goat
7129
5285
1971
1077
507
362
89
84
51
35
23
15
8
5
1
1
1
Clonality
Polyclonal
Conjugate
HRP
2224
1820
1751
1266
793
717
430
343
235
147
145
140
140
116
112
112
102
98
94
93
92
91
89
84
81
80
78
75
73
72
68
68
65
61
55
54
54
54
54
53
53
53
53
53
53
52
48
43
41
37
32
31
23
23
22
20
19
18
17
16
15
15
15
14
14
14
12
12
12
12
12
11
11
10
10
10
10
10
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Dot Blot (DB), Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in goat.
Isotype IgG
Specificity IgG (H&L)
Characteristics Anti-Rabbit IgG peroxidase conjugated antibody generated in goat detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm
Antigen
Background Synonyms: Rabbit Antibody peroxidase conjugated, Gt-a-Rabbit peroxidase conjugated IgG, Rabbit proxidase conjugated Antibody in Goat, Rabbit Secondary peroxidase conjugated Antibody, peroxidase.
Application Notes Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Gentamicin sulfate
Handling Advice Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Supplier Images
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN964977) Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN964977) Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs,...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN964977) Western Blot of Goat anti-Rabbit IgG Peroxidase Conjugated Antibody. Lane 1: HeLa Who...
Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).