Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (Atto 425)

Details for Product No. ABIN964980, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
6229
2030
1776
1135
782
648
372
305
34
34
31
15
12
12
12
12
11
7
3
3
1
1
1
1
1
1
1
Reactivity
Rabbit
3078
2913
2380
1662
1509
785
737
489
476
410
401
395
341
298
271
160
159
111
54
34
30
26
16
15
12
10
8
7
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Host
Goat
7129
5285
1971
1077
507
362
89
84
51
35
23
15
8
5
1
1
1
Clonality
Polyclonal
Conjugate
Atto 425
2225
1820
1751
1266
793
717
430
343
235
147
145
140
140
116
112
112
102
98
94
93
92
91
89
84
81
80
78
75
73
72
68
68
65
61
55
54
54
54
54
53
53
53
53
53
53
52
48
43
41
37
32
31
23
23
22
20
19
18
17
16
15
15
15
14
14
14
12
12
12
12
12
11
11
10
10
10
10
10
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunoassay (IA)
Options
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Immunogen Rabbit IgG whole molecule
Isotype IgG
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Characteristics Anti-Rabbit IgG (H&L) conjugated to ATTO 425 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Antigen
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid cycles of freezing and thawing.
Do NOT add Sodium Azide!
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20 °C or below after dilution.
Product is photosensitive and should be protected from light.
Storage -20 °C
Storage Comment Store vial at -20 °C or below prior to opening.
Expiry Date 12 months
Supplier Images
 image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) (ABIN964980) Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425)
 image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) (ABIN964980) Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) (Image 2)
 image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) (ABIN964980) Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) (Image 3)
 image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) (ABIN964980) Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) (Image 4)
Immunofluorescence (IF) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) (ABIN964980) Dylight anddye conjugated antibodies provide high signal and low background for confo...
Immunofluorescence (IF) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) (ABIN964980) DyLight anddye conjugated antibodies provide high signal and low background for confo...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) (ABIN964980) Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs,...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) (ABIN964980) Western Blot of ATTO 425 conjugated Goat anti-Rabbit IgG antibody. Lane 1: Rabbit IgG...