Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (Atto 647N)

Details for Product No. ABIN964991, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
6012
1904
1869
1159
274
267
266
124
33
30
30
15
12
12
12
12
11
7
3
2
1
1
1
1
1
1
1
Reactivity
Rabbit
2740
2674
2191
1495
1370
700
664
447
434
362
359
354
275
258
257
147
115
84
43
26
24
21
12
12
8
7
5
5
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
Host
Goat
6468
4816
1755
887
458
274
87
54
43
35
22
8
6
5
4
1
1
1
Clonality
Polyclonal
Conjugate
Atto 647N
2064
1741
1639
1144
678
644
422
315
171
122
102
102
94
89
88
88
86
84
79
78
74
73
70
68
68
67
65
65
63
55
55
54
54
54
54
54
53
53
53
53
53
48
47
47
41
40
32
31
30
23
23
22
20
19
18
17
16
15
15
15
14
14
13
12
12
12
12
10
10
10
10
10
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
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Immunogen

Immunogen: Rabbit IgG whole molecule

Isotype IgG
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Cross-Reactivity (Details) Pre-Adsorbed
Characteristics Anti-Rabbit IgG (H&L) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Purification Rabbit IgG (H&L) Antibody ATTO 647N was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.  This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.
Labeling Ratio 2.5
Antigen
Background

Synonyms: Goat anti-Rabbit IgG Antibody ATTO647N Conjugation, Goat anti-Rabbit IgG ATTO 647N Conjugated Antibody

Background: Anti-Rabbit IgG (H&L) ATTO 647N Antibody generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Notes

Application Note: Anti-Rabbit IgG (H&L) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Comment

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid cycles of freezing and thawing.
Dilute only prior to immediate use
Product is photosensitive and should be protected from light.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
Expiry Date 12 months
Supplier Images
 image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N) (ABIN964991) Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N)
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N) (ABIN964991) Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs,...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N) (ABIN964991) ATTO 647N conjugated anti rabbit antibody was used to detect anti-Beta Actin antibody...
Product cited in: Truckenbrodt, Viplav, Jähne, Vogts, Denker, Wildhagen, Fornasiero, Rizzoli: "Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission." in: The EMBO journal, Vol. 37, Issue 15, 2019 (PubMed).

Hruska, Henderson, Le Marchand, Jafri, Dalva: "Synaptic nanomodules underlie the organization and plasticity of spine synapses." in: Nature neuroscience, Vol. 21, Issue 5, pp. 671-682, 2019 (PubMed).

Gomes de Castro, Höbartner, Opazo: "Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy." in: PLoS ONE, Vol. 12, Issue 2, pp. e0173050, 2017 (PubMed).

Saka, Vogts, Kröhnert, Hillion, Rizzoli, Wessels: "Correlated optical and isotopic nanoscopy." in: Nature communications, Vol. 5, pp. 3664, 2015 (PubMed).