Rabbit anti-Golden Syrian Hamster IgG (Heavy & Light Chain) Antibody - Preadsorbed
-
- Target See all IgG products
- IgG
- Fragment
- F(ab')2 fragment
-
Binding Specificity
- Heavy & Light Chain
-
Reactivity
- Golden Syrian Hamster
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Golden Syrian Hamster IgG, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
-
Immunogen: Golden Syrian Hamster IgG whole molecule
- Isotype
- IgG
-
-
- Application Notes
-
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- DO NOT FREEZE. Dilute only prior to immediate use.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4 °C before opening. This product is stable at 4 °C as an undiluted liquid.
- Expiry Date
- 12 months
-
- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
-
Synonyms: Rabbit F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Antibody, Rabbit F(ab')2 Anti-Hamster IgG Antibody, Rabbit Fab2 Anti-Hamster IgG Antibody
Background: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
-