Rabbit anti-Sheep IgG (Heavy & Light Chain) Antibody (PE) - Preadsorbed
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- Target See all IgG products
- IgG
- Fragment
- F(ab')2 fragment
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Sheep
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- PE
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Application
- Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- Specificity
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Sheep IgG and Sheep Serum.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Sheep IgG whole molecule
- Isotype
- IgG
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- Application Notes
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Application Note: F(ab')2 Anti-Sheep IgG Phycoerythrin Conjugated Secondary Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
Flow Cytometry Dilution: 1:100 - 1:250
IF Microscopy Dilution: 1:100 - 1:250
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 0.5 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing. - Storage
- RT,4 °C
- Storage Comment
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: Rabbit F(ab')2 Anti-Sheep IgG Phycoerythrin Conjugated Antibody, Rabbit Fab2 Anti-Sheep IgG Antibody Phycoerythrin Conjugation
Background: F(ab')2 Anti-Sheep IgG (H&L) Antibody generated in rabbit detects immunoglobulin g from sheep, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. This F(ab')2 Anti-Sheep IgG Antibody is conjugated to Phycoerythrin.
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