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Donkey anti-Sheep IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin Fab fragment
Catalog No. ABIN965398
  • Target See all IgG products
    IgG
    Fragment
    Fab fragment
    Binding Specificity
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 692
    • 672
    • 559
    • 452
    • 366
    • 239
    • 197
    • 147
    • 137
    • 131
    • 122
    • 98
    • 95
    • 95
    • 92
    • 62
    • 46
    • 33
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Sheep
    Host
    • 1718
    • 1474
    • 536
    • 203
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Donkey
    Clonality
    • 3346
    • 164
    • 1
    Polyclonal
    Conjugate
    • 556
    • 408
    • 406
    • 366
    • 298
    • 276
    • 228
    • 120
    • 99
    • 67
    • 66
    • 62
    • 62
    • 52
    • 48
    • 47
    • 44
    • 43
    • 43
    • 41
    • 39
    • 32
    • 32
    • 32
    • 31
    • 31
    • 30
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 26
    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 14
    • 10
    Biotin
    Application
    • 1836
    • 1788
    • 1787
    • 1498
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 50
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Donkey Serum.
    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Sheep IgG whole molecule

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid cycles of freezing and thawing.
    This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background

    Synonyms: Donkey Fab Anti-Sheep IgG Biotin Conjugated Antibody, Donkey Fab Fragment Anti-Sheep IgG Antibody Biotin Conjugation

    Background: Fab Anti-Sheep IgG (H&L) Antibody generated in donkey detects immunoglobulin g from sheep, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Fab Anti-Sheep IgG Antibody is conjugated to Biotin.

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