CHEK1 antibody

Catalog No. ABIN965885

Product Details anti-CHEK1 Antibody

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Specificity: Chk1 (Ab-317) antibody detects endogenous levels of total Chk1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Chk1 around the phosphorylation site of serine 317 (S-S-SP-Q-P)

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-CHEK1 antibody (ABIN965885)

Zhang, Otterness, Chiang, Xie, Liu, Mercurio, Abraham: "Genotoxic stress targets human Chk1 for degradation by the ubiquitin-proteasome pathway." in: Molecular cell, Vol. 19, Issue 5, pp. 607-18, (2005) (PubMed).

Rocha, Garrett, Campbell, Schumm, Perkins: "Regulation of NF-kappaB and p53 through activation of ATR and Chk1 by the ARF tumour suppressor." in: The EMBO journal, Vol. 24, Issue 6, pp. 1157-69, (2005) (PubMed).

Clarke, Clarke: "DNA-dependent phosphorylation of Chk1 and Claspin in a human cell-free system." in: The Biochemical journal, Vol. 388, Issue Pt 2, pp. 705-12, (2005) (PubMed).

Yu, Chen: "DNA damage-induced cell cycle checkpoint control requires CtIP, a phosphorylation-dependent binding partner of BRCA1 C-terminal domains." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9478-86, (2004) (PubMed).

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHK1 (CHEK1 Products)

Synonyms: An08g10320 antibody, AO090003000441 antibody, CHK1 antibody, chk1 antibody, C85740 antibody, Chk1 antibody, rad27 antibody, id:ibd2720 antibody, zgc:56093 antibody, CHEK1 antibody, checkpoint kinase 1 antibody, serine/threonine-protein kinase chk1 antibody, serine/threonine-protein kinase Chk1 antibody, CAMK/CAMKL/CHK1 protein kinase Chk1 antibody, checkpoint kinase 1 S homeolog antibody, CHEK1 antibody, ANI_1_2436074 antibody, AOR_1_768154 antibody, PTRG_04183 antibody, SJAG_01680 antibody, PAAG_04978 antibody, MCYG_03290 antibody, VDBG_03742 antibody, chek1.S antibody, Chek1 antibody, chek1 antibody

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair