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Granulin antibody (GRN) Primary Antibody

GRN Reactivity: Human ELISA, WB Monoclonal
Pubmed (2)
Catalog No. ABIN966227
$594.00
Plus shipping costs $45.00
0.1 mL
local_shipping Shipping to: United States
Delivery in 4 to 6 Business Days
  • Target
    Reactivity
    Human
    Host
    Please inquire
    Clonality
    Monoclonal
    Conjugate
    This Granulin antibody is un-conjugated
    Application
    ELISA, Western Blotting (WB)
    Specificity
    Ni-NTA purified truncated recombinant GRN expressed in E. Coli strain BL21 (DE3).
    Purification
    Crude ascites.
    Isotype
    IgG1, IgG3, IgM
  • Target
    Alternative Name
    GRN (GRN Antibody Abstract)
    Background
    Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. GRN is expressed in myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. present in inflammatory cells and bone marrow. highest levels in kidney.
    Gene ID
    2986
  • Application Notes
    Western Blot: 1: 500- 1: 2,000
    ELISA: Propose dilution 1: 10,000.
    Determining optimal working dilutions by titration test.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Storage
    -20 °C
  • Lu, Serrero: "Stimulation of PC cell-derived growth factor (epithelin/granulin precursor) expression by estradiol in human breast cancer cells." in: Biochemical and biophysical research communications, Vol. 256, Issue 1, pp. 204-7, 1999 (PubMed).

    Baba, Hoff, Nemoto, Lee, Orth, Arai, Gerton: "Acrogranin, an acrosomal cysteine-rich glycoprotein, is the precursor of the growth-modulating peptides, granulins, and epithelins, and is expressed in somatic as well as male germ cells." in: Molecular reproduction and development, Vol. 34, Issue 3, pp. 233-43, 1993 (PubMed).

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