IKBKB
Reactivity: Human
ELISA, WB, IHC, IF
Host: Rabbit
Monoclonal
3E1
unconjugated
Recombinant Antibody
Application Notes
Western Bloting: 1/500 - 1/2000. ELISA: Propose dilution 1/10000. Not yet tested in other applications. Determining optimal working dilutions by titration test.
Restrictions
For Research Use only
Format
Liquid
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Park, Song, Kim, Son, Lee, Yoon, Kim, Park, Song, Hwang, Jung, Hong: "Cobrotoxin inhibits NF-kappa B activation and target gene expression through reaction with NF-kappa B signal molecules." in: Biochemistry, Vol. 44, Issue 23, pp. 8326-36, (2005) (PubMed).
Kumar, Rajgopal, Pillai, Babu et al.: "Activation of nuclear transcription factor-kappa B is associated with the induction of inhibitory kappa B kinase-beta and involves differential activation of protein kinase C and protein tyrosine ..." in: Neuroscience letters, Vol. 340, Issue 2, pp. 139-42, (2003) (PubMed).
Peet, Li: "IkappaB kinases alpha and beta show a random sequential kinetic mechanism and are inhibited by staurosporine and quercetin." in: The Journal of biological chemistry, Vol. 274, Issue 46, pp. 32655-61, (2000) (PubMed).
Target
IKBKB
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))
IKBKB(Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta, also called IKK2/IKKB), is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK-beta has been shown to activate NF-kB and phosphorylate IKB-alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK-beta is essential for activation of IKK by TNF and IL1. Once activated, IKK-beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK-beta activity can also be regulated by MEKK-1. Synonyms: IKBKB