XRCC6 antibody (AA 506-541)

Catalog No. ABIN966469

Product Details anti-XRCC6 Antibody

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Binding Specificity: AA 506-541

Reactivity: Human, Mouse, Monkey, Xenopus laevis, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This XRCC6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purification: Concentrated. Purified from the ascites fluid by Protein A chromatography

Immunogen: Human placental extract designated as PSE1-PL

Clone: N3H10

Isotype: IgG2b

Application Details

Application Notes: Immunofluorescence
Immunoprecipitation (Native and denatured)(Use Protein A) (Ab 2µg/mg protein lysate)
Western blotting: (0.25-0.5 µg/ml for 2hrs at RT)
Immunohistology (Formalinparaffin)(Ab 1-2g/ml for 30 minute at RT)
Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH 6.0.

Comment:

Cellular Localization: Nuclear.
Recommended Positive Control: BT474, HEP-G-2 or HeLa Cells, Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL.

Buffer: 10mM PBS, pH 7.4, with 0.2% BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-XRCC6 antibody (ABIN966469)

Wang, Chou, Blankson, Satoh, Knuth, Eisenberg, Pisetsky, Reeves: "Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens." in: Molecular biology reports, Vol. 18, Issue 1, pp. 15-28, (1993) (PubMed).

Target Details for XRCC6

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Alternative Name: Ku (p70) (XRCC6 Products)

Synonyms: CTC75 antibody, CTCBF antibody, G22P1 antibody, KU70 antibody, ML8 antibody, TLAA antibody, 70kDa antibody, G22p1 antibody, Ku70 antibody, Kup70 antibody, X-ray repair cross complementing 6 antibody, ATP-dependent DNA helicase II, 70 kDa subunit antibody, X-ray repair complementing defective repair in Chinese hamster cells 6 L homeolog antibody, X-ray repair complementing defective repair in Chinese hamster cells 6 antibody, XRCC6 antibody, Bm1_41430 antibody, xrcc6.L antibody, Xrcc6 antibody

Background: The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Also available without BSA and azide at 1mg/ml.

Molecular Weight: 70kDa

Gene ID: 2547

Pathways: DNA Damage Repair