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Calprotectin antibody

S100A8/A9 Reactivity: Human, Mouse, Baboon, Cat, Dog, Guinea Pig, Monkey, Pig, Rabbit, Rat IHC (p), IHC (fro) Host: Mouse Monoclonal B314-1 (MAC 387) unconjugated
Catalog No. ABIN966510
  • Target See all Calprotectin (S100A8/A9) Antibodies
    Calprotectin (S100A8/A9)
    Reactivity
    Human, Mouse, Baboon, Cat, Dog, Guinea Pig, Monkey, Pig, Rabbit, Rat
    Host
    • 24
    • 7
    Mouse
    Clonality
    • 24
    • 7
    Monoclonal
    Conjugate
    • 16
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    This Calprotectin antibody is un-conjugated
    Application
    • 17
    • 12
    • 12
    • 10
    • 6
    • 6
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    This antibody reacts with macrophage L1 protein, also known as calprotectin, calgranulin, or cystic fibrosis antigen.
    Purification
    Concentrated.
    Immunogen
    Affinity purified monocyte membrance preparation.
    Clone
    B314-1 (MAC 387)
    Isotype
    IgG1
    Top Product
    Discover our top product S100A8/A9 Primary Antibody
  • Application Notes
    Immunohistochemistry: 1:50-1:100.
    Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. This antibody may be used at a dilution 1:50-1:100 in IHC. When using routinely processed tissues, predigestion with trypsin @ 1 mg/ml PBS, 10 mins. at 37 C. or a high temperature antigenic unmasking technique (boiling tissue sections in 10 mM citrate, pH 6.0 for 10-20 mins., folowed by cooling to RT for 10-20 mins.) is required. The optimal conditions should be determined by the individual laboratory.
    Comment

    Cellular Localization: cytoplasmic.
    Recommended Positive Control: Tonsil, Lymph Node or Spleen

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 mg/ml.
    Buffer
    20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Andersen, Ladefoged, Larsen: "Acute kidney graft rejection. A morphological and immunohistological study on "zero-hour" and follow-up biopsies with special emphasis on cellular infiltrates and adhesion molecules." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 102, Issue 1, pp. 23-37, (1994) (PubMed).

  • Target
    Calprotectin (S100A8/A9)
    Alternative Name
    Macrophage/L 1 Protein/Calprotectin (S100A8/A9 Products)
    Synonyms
    60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, Mrp14 antibody, MRP-14 antibody, p14 antibody, 60B8Ag antibody, AW546964 antibody, BEE22 antibody, Cagb antibody, GAGB antibody, L1Ag antibody, S100 calcium binding protein A9 antibody, S100 calcium binding protein A9 (calgranulin B) antibody, S100A9 antibody, S100a9 antibody
    Gene ID
    3552
    Pathways
    S100 Proteins
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