N-Cadherin antibody (Middle Region)

Catalog No. ABIN966637

Product Details anti-N-Cadherin Antibody

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Cadherin-2 (CDH2) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human N-Cadherin, identical to the related rat and mouse sequences.< br/>Immunogen was affinity purified.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

References for anti-N-Cadherin antibody (ABIN966637)

Togashi, Abe, Mizoguchi, Takaoka, Chisaka, Takeichi: "Cadherin regulates dendritic spine morphogenesis." in: Neuron, Vol. 35, Issue 1, pp. 77-89, (2002) (PubMed).

Miyatani, Copeland, Gilbert, Jenkins, Takeichi: "Genomic structure and chromosomal mapping of the mouse N-cadherin gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8443-7, (1992) (PubMed).

Walsh, Barton, Putt, Moore, Kelsell, Spurr, Goodfellow: "N-cadherin gene maps to human chromosome 18 and is not linked to the E-cadherin gene." in: Journal of neurochemistry, Vol. 55, Issue 3, pp. 805-12, (1990) (PubMed).

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: Cadherin-2 (CDH2 Products)

Synonyms: CD325 antibody, CDHN antibody, CDw325 antibody, NCAD antibody, N-cadherin antibody, cadherin-2 antibody, cdhn antibody, ncad antibody, cdw325 antibody, CDH2 antibody, Cadherin-2 antibody, Ncad antibody, glo antibody, lyr antibody, pac antibody, wu:fb47h04 antibody, cadherin 2 antibody, cadherin 2 S homeolog antibody, cadherin 2, type 1, N-cadherin (neuronal) antibody, CDH2 antibody, cdh2.S antibody, cdh2 antibody, Cdh2 antibody

Background: N-cadherin (NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). N-Cadherin, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96 % identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene (including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11. 2. Cadherin regulates dendritic spine morphogenesis.

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane