NOS2 antibody (C-Term)

Catalog No. ABIN966678

Product Details anti-NOS2 Antibody

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Nitric oxide synthase, inducible (NOS2) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human NOS2, identical to the related rat and mouse sequences.< br/>Immunogen was affinity purified.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

References for anti-NOS2 antibody (ABIN966678)

Hobbs, Udhayakumar, Levesque, Booth, Roberts, Tkachuk, Pole, Coon, Kariuki, Nahlen, Mwaikambo, Lal, Granger, Anstey, Weinberg: "A new NOS2 promoter polymorphism associated with increased nitric oxide production and protection from severe malaria in Tanzanian and Kenyan children." in: Lancet (London, England), Vol. 360, Issue 9344, pp. 1468-75, (2002) (PubMed).

Diefenbach, Schindler, Röllinghoff, Yokoyama, Bogdan: "Requirement for type 2 NO synthase for IL-12 signaling in innate immunity." in: Science (New York, N.Y.), Vol. 284, Issue 5416, pp. 951-5, (1999) (PubMed).

Chartrain, Geller, Koty, Sitrin, Nussler, Hoffman, Billiar, Hutchinson, Mudgett: "Molecular cloning, structure, and chromosomal localization of the human inducible nitric oxide synthase gene." in: The Journal of biological chemistry, Vol. 269, Issue 9, pp. 6765-72, (1994) (PubMed).

Target Details for NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Alternative Name: Nitric oxide synthase, inducible (NOS2 Products)

Synonyms: HEP-NOS antibody, INOS antibody, NOS antibody, NOS2A antibody, NOS-II antibody, Nos-2 antibody, Nos2a antibody, i-NOS antibody, iNOS antibody, iNos antibody, NOS2a antibody, NOS2 antibody, BmNOS2 antibody, Nsl antibody, nos2 antibody, nitric oxide synthase 2 antibody, nitric oxide synthase 2, inducible antibody, inducible nitric oxide synthase antibody, inducible nitric-oxide synthase antibody, nanos C2HC-type zinc finger 2 antibody, NOS2 antibody, Nos2 antibody, nos2 antibody, LOC396821 antibody, NANOS2 antibody

Background: Nitric oxide synthase, inducible is an enzyme that in humans is encoded by the NOS2 gene. Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter, it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. Three different NOS isoforms have been identified which fall into two distinct types, constitutive and inducible. The inducible NOS (iNOS) isoform is expressed in a variety of cell types and tissues in response to inflammatory agents and cytokines. The human iNOS (NOS2) gene is approximately 37 kb in length and consists of 26 exons and 25 introns. Diefenbach et al. (1999) studied the relationship of IL12 and nitric oxide synthase-2 (NOS2) to innate immunity to the parasite Leishmania in mice. And conclude that NOS2-derived NO is a prerequisite for cytokine signaling and function in innate immunity. From studies in Tanzania and Kenya, Hobbs et al. (2002) identified a novel single-nucleotide polymorphism, -1173C-T (163730. 0001), in the NOS2 promoter that was significantly associated with protection from symptomatic malaria and severe malarial anemia.

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process