p105 Proliferating-Associated Nuclear Antigen (Nuclear) antibody
Reactivity: Human
IHC (fp), WB
Host: Mouse
Monoclonal
B301
unconjugated
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- Target
- p105 Proliferating-Associated Nuclear Antigen
- Binding Specificity
- Nuclear
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)
- Specificity
- The p105 protein, found in all human cells in a dimeric form (105 kD) and monomeric form (41 kD), is a proliferation-associated nuclear antigen that is absent in non-cycling cells in the G0 phase. This protein is important in the synthesis and transport of RNA in the cell regulation cycle. During G2 and mitosis, this protein is increased possibly due to the unmasking of nucleolar antigens, resulting in large accumulations of this protein in the mitotic cytoplasm.
- Purification
- Concentrated.
- Immunogen
- Activated peripheral blood nuclei.
- Clone
- B301
- Isotype
- IgM
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- Application Notes
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Immunohistochemistry: 1:50-1:100. Formalin-fixed paraffin-embedded (FFPE) tissue sections require pepsin or trypsin digestion.
Western blotting: 1:100-1:200 .
Staining Procedure: This antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. FFPE tissue sections require pepsin or trypsin digestion. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory. - Comment
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Cellular Localization: Nuclear.
Recommended Positive Control: Human tonsil or lymph node. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/ml.
- Buffer
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- p105 Proliferating-Associated Nuclear Antigen
- Molecular Weight
- Dimeric form 105 kDa, Monomeric form 41 kDa
- Gene ID
- 4790
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