WNT5A antibody

Catalog No. ABIN967259

Product Details anti-WNT5A Antibody

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This WNT5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human XBP1 expressed in E. Coli.

Clone: 6F2

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-WNT5A antibody (ABIN967259)

Martino, Olsen, Fulcher, Wolfgang, ONeal, Ribeiro: "Airway epithelial inflammation-induced endoplasmic reticulum Ca2+ store expansion is mediated by X-box binding protein-1." in: The Journal of biological chemistry, Vol. 284, Issue 22, pp. 14904-13, (2009) (PubMed).

Schardt, Weber, Eyholzer, Mueller, Pabst: "Activation of the unfolded protein response is associated with favorable prognosis in acute myeloid leukemia." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 11, pp. 3834-41, (2009) (PubMed).

Target Details for WNT5A

Target: WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))

Alternative Name: WNT5A (WNT5A Products)

Synonyms: hWNT5A antibody, wnt-5A antibody, wnt5a-B antibody, WNT5A antibody, 8030457G12Rik antibody, Wnt-5a antibody, Wnt family member 5A antibody, Wnt family member 5A S homeolog antibody, wingless-type MMTV integration site family, member 5a antibody, wingless-type MMTV integration site family, member 5A antibody, wingless-related MMTV integration site 5A antibody, Wnt signaling ligand antibody, WNT5A antibody, Wnt5a antibody, wnt5a.S antibody, wnt5a antibody, LOC578814 antibody, wnt5 antibody

Background: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
Synonyms: hWNT5A

Molecular Weight: 42.3kDa

Gene ID: 7474

Pathways: WNT Signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation