BCL2L1 antibody (AA 3-14)

Catalog No. ABIN967301

Product Details anti-BCL2L1 Antibody

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Binding Specificity: AA 3-14

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCL2L1 antibody is un-conjugated

Application: Western Blotting (WB)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human, mouse Bcl-xL / Bcl-xS (aa. 3-14)

Clone: 2H12

Isotype: IgG2a

Application Details

Application Notes: In addition to Jurkat cell lysates, MOLT-4 (Human T-lymphoblasts, ATCC CRL-1582), K-562 (Human bone marrow myelogenous leukemia, ATCC CCL-243), Hut-78 (Human T lymphoma, ATCC TIB-161), human peripheral blood mononuclear cells (PBMC), rat thymocytes and mouse thymocytes have also been reported to be useful as positive controls.

Comment:

Related Products: ABIN968537

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-BCL2L1 antibody (ABIN967301)

Hsu, Wolter, Youle: "Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3668-72, (1997) (PubMed).

Hsu, Youle: "Nonionic detergents induce dimerization among members of the Bcl-2 family." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13829-34, (1997) (PubMed).

Reed, Miyashita, Krajewski, Takayama, Aime-Sempe, Kitada, Sato, Wang, Harigai, Hanada, Krajewska, Kochel, Millan, Kobayashi: "Bcl-2 family proteins and the regulation of programmed cell death in leukemia and lymphoma." in: Cancer treatment and research, Vol. 84, pp. 31-72, (1996) (PubMed).

Krajewski, Krajewska, Shabaik, Wang, Irie, Fong, Reed: "Immunohistochemical analysis of in vivo patterns of Bcl-X expression." in: Cancer research, Vol. 54, Issue 21, pp. 5501-7, (1994) (PubMed).

Target Details for BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Alternative Name: Bcl-x (BCL2L1 Products)

Synonyms: BCL-XL/S antibody, BCL2L antibody, BCLX antibody, BCLXL antibody, BCLXS antibody, Bcl-X antibody, PPP1R52 antibody, bcl-xL antibody, bcl-xS antibody, Bcl(X)L antibody, Bcl-XL antibody, Bcl-Xs antibody, Bcl2l antibody, BclX antibody, bcl-x antibody, Bcl-xl antibody, Bclx antibody, bcl-X antibody, Bcl-xL antibody, BCL-XL antibody, BCL2L1 antibody, bcl-XL antibody, bcl-x(l) antibody, bcl-xl/s antibody, bcl-xs antibody, bcl2l antibody, bclx antibody, bclxl antibody, xR11 antibody, bcl-xl antibody, blp1 antibody, cb1021 antibody, zfBLP1 antibody, BCL2 like 1 antibody, BCL2-like 1 antibody, Bcl2-like 1 antibody, BCL2-like 1 L homeolog antibody, bcl2-like 1 antibody, BCL2L1 antibody, Bcl2l1 antibody, bcl2l1 antibody, bcl2l1.L antibody

Background: Members of the Bcl-2 family play a major role in regulating the response of cells to a wide variety of apoptotic signals. Some Bcl-2 family members like Bcl-2, block apoptosis, whereas others promote apoptosis and inhibit Bcl-2 activity. Bcl-x is a Bcl-2 homologue that has two isoforms, resulting from alternative splicing. Bcl-xL (long) is a 241 amino acid protein (25-29 kDa) that is 47% homologous to Bcl-2 on the amino acid level. Bcl-xS (short) is a 178 amino acid protein (~ 19.5 kDa) lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 protein family. Bcl-xL blocks cell death, whereas Bcl-xS inhibits Bcl-2 and promotes cell death. The 2H12 antibody has been reported to recognize human, mouse and rat Bcl-xL (long) protein. An N-terminal peptide (amino acids 3-14) common to human and mouse Bcl-xL and Bcl-xS (short) proteins was used as the immunogen. Thus the antibody is also predicted to recognize the Bcl-xS protein as the sequence used for the immunogen is common to both Bcl-xL and Bcl-xS proteins. Reports from development, however, have indicated that Bcl-xS has not been observable.

Molecular Weight: 25-29 kDa

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling