tau antibody

Catalog No. ABIN967493

Product Details anti-tau Antibody

Target: tau (tau Protein)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This tau antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: TAU-5

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-tau antibody (ABIN967493)

Papasozomenos, Su: "Rapid dephosphorylation of tau in heat-shocked fetal rat cerebral explants: prevention and hyperphosphorylation by inhibitors of protein phosphatases PP1 and PP2A." in: Journal of neurochemistry, Vol. 65, Issue 1, pp. 396-406, (1995) (PubMed).

Riederer, Binder: "Differential distribution of tau proteins in developing cat cerebellum." in: Brain research bulletin, Vol. 33, Issue 2, pp. 155-61, (1994) (PubMed).

Watanabe, Takio, Hasegawa, Arai, Titani, Ihara: "Tau 2: a probe for a Ser conformation in the amino terminus of tau." in: Journal of neurochemistry, Vol. 58, Issue 3, pp. 960-6, (1992) (PubMed).

Ksiezak-Reding, Binder, Yen: "Alzheimer disease proteins (A68) share epitopes with tau but show distinct biochemical properties." in: Journal of neuroscience research, Vol. 25, Issue 3, pp. 420-30, (1990) (PubMed).

Papasozomenos, Binder: "Phosphorylation determines two distinct species of Tau in the central nervous system." in: Cell motility and the cytoskeleton, Vol. 8, Issue 3, pp. 210-26, (1988) (PubMed).

Target Details for tau

Target: tau (tau Protein)

Alternative Name: Tau (tau Products)

Synonyms: DDPAC antibody, FTDP-17 antibody, MAPTL antibody, MSTD antibody, MTBT1 antibody, MTBT2 antibody, PPND antibody, TAU antibody, tau antibody, PHF-tau antibody, microtubule associated protein tau antibody, MAPT antibody

Background: Tau proteins are microtuble-associated proteins (MAPs) that are thought to function by stabilizing microtubles. They comprise a family of multiple polypeptides that originate from one gene. Alternate splicing of Tau mRNA and differential phosphorylation contribute to the heterogeneity of Tau. Typically, Tau proteins migrate between ~ 45-70 kDa with isoelectric points ranging from 6.5-8.0. Tau expression is widespread in the brain and is found in most cell types within the mammalian central nervous system. Tau has a complex biochemical nature that is partly due to its many isoforms. The number and molecular weights of Tau forms changes during brain development. Additionally, the molecular wieghts and forms of Tau proteins at a given point of development vary between species. Antibodies directed against different epitopes have been used to distinguish between Tau forms. The monoclonal antibody TAU-5 recognizes a phosphorylation-independent epitope on Tau proteins. The reduced molecular weight of Tau proteins may range between 45 kDa to 95 kDa. The TAU-5 antibody reacts with a phosphorylation-independent epitope in the middle of tau. It recognizes both the phosphorylated and non-phosphorylated form of Tau. The antibody has been characterized in rat tissue.

Molecular Weight: 45-70 kDa