BAK1 antibody

Catalog No. ABIN967519

Product Details anti-BAK1 Antibody

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAK1 antibody is un-conjugated

Application: Western Blotting (WB)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Bak

Clone: G317-2

Isotype: IgG1

Application Details

Application Notes: Applications include western blot analysis (1-2 µg/ml). HeLa cells (ATCC CCL-2) are recommended as a positive control. HeLa cell lysate is also available as a ready-to-use positive western blot control (ABIN968535).

Comment:

Related Products: ABIN967389, ABIN968535

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-BAK1 antibody (ABIN967519)

Chittenden, Harrington, OConnor, Flemington, Lutz, Evan, Guild: "Induction of apoptosis by the Bcl-2 homologue Bak." in: Nature, Vol. 374, Issue 6524, pp. 733-6, (1995) (PubMed).

Kiefer, Brauer, Powers, Wu, Umansky, Tomei, Barr: "Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak." in: Nature, Vol. 374, Issue 6524, pp. 736-9, (1995) (PubMed).

Reed: "Bcl-2 and the regulation of programmed cell death." in: The Journal of cell biology, Vol. 124, Issue 1-2, pp. 1-6, (1994) (PubMed).

Yin, Oltvai, Korsmeyer: "BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax." in: Nature, Vol. 369, Issue 6478, pp. 321-3, (1994) (PubMed).

Target Details for BAK1

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Alternative Name: Bak (BAK1 Products)

Synonyms: BAK antibody, BAK-LIKE antibody, BCL2L7 antibody, CDN1 antibody, Bak antibody, N-BAK1 antibody, N-Bak antibody, Bak1 antibody, bak antibody, bak1 antibody, BCL2 antagonist/killer 1 antibody, BCL2-antagonist/killer 1 antibody, BCL2 antagonist/killer 1 L homeolog antibody, BAK1 antibody, Bak1 antibody, bak1.L antibody

Background: Bak (for Bcl-2 homologous Antagonist/Killer) is a recently identified member of the Bcl-2 family. Bcl-2 family members are involved in mediating programmed cell death or apoptosis, and share two highly conserved functional regions, Bcl-2 homology 1 and 2 (BH1 and BH2). Several of the family members, including Bcl-2, act as inhibitors of apoptosis, whereas others such as Bax promote cell death. Like Bax promote cell death. Like Bax, Bak primarily promotes apoptosis. However, unlike Bax, Bak has also been shown to inhibit cell death. Bak inhibited both serum-starvation and drug induced apoptosis when overexpressed in an Epstein-Barr virus (EBV)-transformed cell line. However, Bax did not inhibit cell death under the same conditions. Bak mNRA has been identified in a wide variety of fetal and adult tissues, with the highest levels observed in heart and skeletal muscle. The apparently ubiquitous expression of Bak has lead to the suggestion that its function may be mediated by cell death inhibitory factors, particularly in cell types with a long life span. Bak migrates at a reduced molecular weight ~24 kDa. G317-2 reacts with human Bak. A synthetic peptide corresponding to the first 50 amino acids of human Bak was used as immunogen.

Molecular Weight: 24 kDa

Pathways: Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response