NME1 antibody (Non-Metastatic Cells 1, Protein (NM23A) Expressed in) (AA 44-152)

Details for Product anti-NME1 Antibody No. ABIN967854, Supplier: Log in to see
Antigen
  • AWD
  • GAAD
  • NB
  • NBS
  • NDKA
  • NDPK-A
  • NDPKA
  • NM23
  • NM23-H1
  • AL024257
  • NM23-M1
  • NM23A
  • NDPK-Z1
  • NM23-B
  • NM23-Z1
  • ndpkz1
  • nm23b
  • nme1
  • nme2
  • nme2b1
  • NME1
  • NME1-NME2
  • NM23-C1
  • ndpk-b
  • ndpkb
  • nm23-h2
  • nm23ndk
  • puf
  • NBR-A
  • NBR-B
  • NME1-1
  • nm23ndk-a
  • NDK I
  • NDPK I
  • T30A10.80
  • T30A10_80
  • NDP kinase I
  • NME/NM23 nucleoside diphosphate kinase 1
  • NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1
  • non-metastatic cells 1, protein (NM23A) expressed in
  • NME/NM23 nucleoside diphosphate kinase 2 S homeolog
  • NME/NM23 nucleoside diphosphate kinase 2 L homeolog
  • nucleoside diphosphate kinase
  • nucleoside diphosphate kinase 1
  • NME1
  • Nme1
  • nme2b.1
  • nme2.S
  • nme2.L
  • LOC547870
  • NDPK1
  • LOC107790563
Alternatives
anti-Human NME1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 44-152
24
20
13
13
10
9
9
9
7
7
6
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
290
52
49
14
9
8
6
4
4
3
2
2
2
1
1
Host
Mouse
145
140
6
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This NME1 antibody is un-conjugated
12
12
8
7
6
6
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
250
128
86
65
38
14
13
11
10
7
6
2
2
1
Options
Supplier
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Supplier Product No.
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Immunogen Human Nm23 aa. 44-152
Clone 56-Nm23
Isotype IgG2b
Cross-Reactivity Mouse (Murine), Rat (Rattus), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Nm23 (NME1 Antibody Abstract)
Background Nm23 is a potential metastasis suppressor protein whose expression is either reduced, altered (by mutation), or amplified in various types of metastatic carcinomas. Two human gene homologues, nm23-H1 and nm23-H2, encode 17 kDa proteins that are 90% identical. Nm23 proteins possess a nucleoside diphosphate kinase (NDPK) activity. This enzymatic activity catalyzes the synthesis of non-adenine-containing nucleoside triphosphates from nucleoside diphosphates via a phosphorylated enzyme intermediate. In addition, Nm23 inhibits differentiation, interacts with GTP-binding (GAP) proteins, autophosphorylates serine residues, and binds to DNA. The biochemical mechanisms by which Nm23 affects tumor metastatic potential have yet to be determined. In murine melanoma cell lines, serine 44 is the major site of autophosphorylation on Nm23-1. This acid-stable phosphorylation of Nm23 is inhibited in vitro by cAMP and in vivo by forskolin. These data indicate that this serine phosphorylation is regulated via some cAMP-dependent event in signal transduction. In addition, it has been shown that the Nm23-H2 protein is identical to the c-myc transcription factor PuF. This suggests that some of the cellular effects of Nm23 are mediated by its transcriptional regulatory function, while others are mediated by its NDPK activity.
Molecular Weight 17 kDa
Pathways Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 44-152) antibody (ABIN967854) Western blot analysis of Nm23 on a HeLa cell lysate (Human cervical epitheloid carcin...
Immunofluorescence (IF) image for anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 44-152) antibody (ABIN967854) Immunofluorescence staining of human intestinal smooth muscle cells (HISM).
Product cited in: MacDonald, De la Rosa, Benedict, Freije, Krutsch, Steeg: "A serine phosphorylation of Nm23, and not its nucleoside diphosphate kinase activity, correlates with suppression of tumor metastatic potential." in: The Journal of biological chemistry, Vol. 268, Issue 34, pp. 25780-9, 1994 (PubMed).

Postel, Ferrone: "Nucleoside diphosphate kinase enzyme activity of NM23-H2/PuF is not required for its DNA binding and in vitro transcriptional functions." in: The Journal of biological chemistry, Vol. 269, Issue 12, pp. 8627-30, 1994 (PubMed).

Rosengard, Krutzsch, Shearn, Biggs, Barker, Margulies, King, Liotta, Steeg: "Reduced Nm23/Awd protein in tumour metastasis and aberrant Drosophila development." in: Nature, Vol. 342, Issue 6246, pp. 177-80, 1989 (PubMed).

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