Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Ezrin antibody (AA 391-515)

EZR Reactivity: Human, Dog WB, IHC, IP, IF Host: Mouse Monoclonal 18-Ezrin unconjugated
Catalog No. ABIN968058
  • Target See all Ezrin (EZR) Antibodies
    Ezrin (EZR)
    Binding Specificity
    • 29
    • 16
    • 12
    • 11
    • 9
    • 9
    • 9
    • 8
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 391-515
    Reactivity
    • 154
    • 81
    • 74
    • 8
    • 7
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Human, Dog
    Host
    • 142
    • 11
    • 3
    • 2
    Mouse
    Clonality
    • 138
    • 19
    Monoclonal
    Conjugate
    • 90
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This Ezrin antibody is un-conjugated
    Application
    • 118
    • 54
    • 26
    • 17
    • 16
    • 13
    • 13
    • 13
    • 10
    • 9
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)
    Cross-Reactivity
    Dog (Canine)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human Ezrin aa. 391-515
    Clone
    18-Ezrin
    Isotype
    IgG1
    Top Product
    Discover our top product EZR Primary Antibody
  • Comment

    Related Products: ABIN968619, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Perez, Kinoshita, Hitoshi, Payan, Kitamura, Nolan, Lorens: "Activation of the PKB/AKT pathway by ICAM-2." in: Immunity, Vol. 16, Issue 1, pp. 51-65, (2002) (PubMed).

    Anastasiadis, Moon, Thoreson, Mariner, Crawford, Zheng, Reynolds: "Inhibition of RhoA by p120 catenin." in: Nature cell biology, Vol. 2, Issue 9, pp. 637-44, (2001) (PubMed).

    Defacque, Egeberg, Habermann, Diakonova, Roy, Mangeat, Voelter, Marriott, Pfannstiel, Faulstich, Griffiths: "Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes." in: The EMBO journal, Vol. 19, Issue 2, pp. 199-212, (2000) (PubMed).

    Mohler, Kreda, Boucher, Sudol, Stutts, Milgram: "Yes-associated protein 65 localizes p62(c-Yes) to the apical compartment of airway epithelia by association with EBP50." in: The Journal of cell biology, Vol. 147, Issue 4, pp. 879-90, (1999) (PubMed).

    Berryman, Gary, Bretscher: "Ezrin oligomers are major cytoskeletal components of placental microvilli: a proposal for their involvement in cortical morphogenesis." in: The Journal of cell biology, Vol. 131, Issue 5, pp. 1231-42, (1996) (PubMed).

  • Target
    Ezrin (EZR)
    Alternative Name
    Ezrin (EZR Products)
    Synonyms
    CVIL antibody, CVL antibody, VIL2 antibody, ezr antibody, vil2 antibody, wu:fc05c03 antibody, wu:fj31d06 antibody, cvil antibody, cvl antibody, Vil2 antibody, AW146364 antibody, R75297 antibody, p81 antibody, ezr.S antibody, ezrin antibody, ezrin b antibody, ezrin L homeolog antibody, EZR antibody, ezrb antibody, ezr antibody, Ezr antibody, ezr.L antibody
    Background
    First described as an 80kDa protein concentrated in the apical cytoskeletal region of intestinal brush border cells, ezrin is now recognized as a major substrate of protein tyrosine kinases, such as the epidermal growth factor (EGF) tyrosine kinase. Ezrin is expressed at high levels in intestine, kidney, and placenta. In placenta, ezrin is present as monomers and non-covalent oligomers in tight association with actin microfilaments. In the human epidermoid carcinoma cell line A431, microvilli-like structures appear within 30 seconds after the addition of EGF. These structures give way to membrane ruffles after 2-5 minutes, followed by cell rounding after 10-20 minutes. At the same time, ezrin is recruited into these structures and oligomers are formed following its tyrosine phosphorylation. It is thought that tyrosine phosphorylation triggers the formation of ezrin oligomers.
    Molecular Weight
    80 kDa
    Pathways
    Maintenance of Protein Location
You are here:
Support