AP4E1 antibody (AA 685-793)

Catalog No. ABIN968712

Product Details anti-AP4E1 Antibody

Target: AP4E1 (Adaptor-Related Protein Complex 4, epsilon 1 Subunit (AP4E1))

Binding Specificity: AA 685-793

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AP4E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Adaptin epsilon aa. 685-793

Clone: 32-Adaptin epsilon

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-AP4E1 antibody (ABIN968712)

Aguilar, Boehm, Gorshkova, Crouch, Tomita, Saito, Ohno, Bonifacino: "Signal-binding specificity of the mu4 subunit of the adaptor protein complex AP-4." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 13145-52, (2001) (PubMed).

DellAngelica, Mullins, Bonifacino: "AP-4, a novel protein complex related to clathrin adaptors." in: The Journal of biological chemistry, Vol. 274, Issue 11, pp. 7278-85, (1999) (PubMed).

Hirst, Bright, Rous, Robinson: "Characterization of a fourth adaptor-related protein complex." in: Molecular biology of the cell, Vol. 10, Issue 8, pp. 2787-802, (1999) (PubMed).

Target Details for AP4E1

Target: AP4E1 (Adaptor-Related Protein Complex 4, epsilon 1 Subunit (AP4E1))

Alternative Name: Adaptin epsilon (AP4E1 Products)

Synonyms: CPSQ4 antibody, SPG51 antibody, RGD1562199 antibody, 2310033A20Rik antibody, 9930028M04Rik antibody, AV087807 antibody, adaptor related protein complex 4 epsilon 1 subunit antibody, adaptor-related protein complex 4, epsilon 1 subunit antibody, adaptor-related protein complex AP-4, epsilon 1 antibody, AP4E1 antibody, Ap4e1 antibody

Background: Sorting of integral membrane proteins is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large adaptin proteins (gamma, alpha, sigma, epsilon and beta1, beta2, beta3, beta4 respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, µ4 respectively) and one small chain (sigma1, sigma2, sigma3, sigma4 respectively). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. AP-4 is associated with non-clathrin coated vesicles in the region of the TGN. This localization is disrupted by brefeldin A, indicating that AP-4 membrane attachment is regulated by small GTPases. The µ4 subunit of the AP-4 complex interacts with tyrosine-based signals on LAMP-2 during targeting to the endosomal-lysosomal system. Thus, AP-4 is a less abundant AP complex that may be important for vesicle trafficking from the Golgi to the endosomal-lysosomal system.

Molecular Weight: 127 kDa