GBF1 antibody (AA 1266-1379)

Catalog No. ABIN968745

Product Details anti-GBF1 Antibody

Target: GBF1 (Golgi Brefeldin A Resistant Guanine Nucleotide Exchange Factor 1 (GBF1))

Binding Specificity: AA 1266-1379

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GBF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human GBF1 aa. 1266-1379

Clone: 25-GBF1

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-GBF1 antibody (ABIN968745)

Claude, Zhao, Kuziemsky, Dahan, Berger, Yan, Armold, Sullivan, Melançon: "GBF1: A novel Golgi-associated BFA-resistant guanine nucleotide exchange factor that displays specificity for ADP-ribosylation factor 5." in: The Journal of cell biology, Vol. 146, Issue 1, pp. 71-84, (1999) (PubMed).

Mansour, Herbrick, Scherer, Melançon: "Human GBF1 is a ubiquitously expressed gene of the sec7 domain family mapping to 10q24." in: Genomics, Vol. 54, Issue 2, pp. 323-7, (1999) (PubMed).

Target Details for GBF1

Target: GBF1 (Golgi Brefeldin A Resistant Guanine Nucleotide Exchange Factor 1 (GBF1))

Alternative Name: GBF1 (GBF1 Products)

Synonyms: ARF1GEF antibody, MGC148393 antibody, DKFZp459D2411 antibody, arf1gef antibody, 1700083E03Rik antibody, AI035702 antibody, AW049900 antibody, mKIAA0248 antibody, RGD1307160 antibody, golgi brefeldin A resistant guanine nucleotide exchange factor 1 antibody, Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 antibody, golgi-specific brefeldin A-resistance factor 1 antibody, GBF1 antibody, LOC100185387 antibody, gbf1 antibody, Gbf1 antibody

Background: The Golgi apparatus is a complex and dynamic organelle that functions in protein sorting and modification. Numerous structural and regulatory proteins are involved in the budding, docking, and fusion of Golgi-directed vesicles. Golgi-specific Brefeldin A (BFA)-resistance Factor (GBF1) is a guanine nucleotide exchange factor that contains a centrally located Sec7 domain, and a proline-rich region at the C-terminus. The Sec7 domain is commonly found in a variety of secretory proteins, and is required for activation of ARFs. GBF1 mRNA is ubiquitously expressed, and GBF1 protein localizes to the Golgi, as well as to perinuclear structures that contain COPI. BFA is a fungal heterocyclic lactone that disrupts membrane recruitment of ARFs. This action blocks protein secretion, and changes the morphology of various organelles, such as the Golgi. Overexpression of GBF1 suppresses BFA-induced changes in Golgi morphology, ARF activation, and coat protein recruitment. GBF1 interacts preferentially with ARF5 in the presence of Mg2+. Thus, GBF1 is a Golgi-specific guanine nucleotide exchange factor that activates ARFs through a mechanism that interferes with BFA action.
Synonyms: Golgi-specific Brefeldin A (BFA)-resistance Factor 1

Molecular Weight: 206 kDa