PSMB8 antibody

Catalog No. ABIN969371

Product Details anti-PSMB8 Antibody

Target: PSMB8 (Proteasome (Prosome, Macropain) Subunit, beta Type, 8 (Large Multifunctional Peptidase 7) (PSMB8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PSMB8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Purpose: PSMB8 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human PSMB8 expressed in E. Coli.

Clone: 1A5

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-PSMB8 antibody (ABIN969371)

Camarena, Aquino-Galvez, Falfán-Valencia, Sánchez, Montaño, Ramos, Juárez, García-de-Alba, Granados, Selman: "PSMB8 (LMP7) but not PSMB9 (LMP2) gene polymorphisms are associated to pigeon breeder's hypersensitivity pneumonitis." in: Respiratory medicine, Vol. 104, Issue 6, pp. 889-94, (2010) (PubMed).

Mehta, Jordanova, Corver, van Wezel, Uh, Kenter, Jan Fleuren: "Single nucleotide polymorphisms in antigen processing machinery component ERAP1 significantly associate with clinical outcome in cervical carcinoma." in: Genes, chromosomes & cancer, Vol. 48, Issue 5, pp. 410-8, (2009) (PubMed).

Target Details for PSMB8

Target: PSMB8 (Proteasome (Prosome, Macropain) Subunit, beta Type, 8 (Large Multifunctional Peptidase 7) (PSMB8))

Alternative Name: PSMB8 (PSMB8 Products)

Background:

Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified, both isoforms are processed to yield the same mature subunit.

Aliases: JMP, LMP7, D6S216, PSMB5i, RING10, D6S216E, MGC1491

Molecular Weight: 30kDa

Gene ID: 5696

HGNC: 5696

UniProt: P28062

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA