XRCC5 antibody

Catalog No. ABIN969463

This Mouse Monoclonal antibody specifically detects XRCC5 in WB, IHC, ELISA, ICC and FACS. It exhibits reactivity toward Human and Mouse and has been mentioned in 2+ publications.

Quick Overview for XRCC5 antibody (ABIN969463)

Target: XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This XRCC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 5C5

Product Details anti-XRCC5 Antibody

Purpose: XRCC5 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human XRCC5 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-XRCC5 antibody (ABIN969463)

Nolens, Pignon, Koopmansch, Elmoualij, Zorzi, De Pauw, Winkler: "Ku proteins interact with activator protein-2 transcription factors and contribute to ERBB2 overexpression in breast cancer cell lines." in: Breast cancer research : BCR, Vol. 11, Issue 6, pp. R83, (2010) (PubMed).

Ohno, Kunimoto, Nishizuka, Osada, Imagawa: "Ku proteins function as corepressors to regulate farnesoid X receptor-mediated gene expression." in: Biochemical and biophysical research communications, Vol. 390, Issue 3, pp. 738-42, (2009) (PubMed).

Target Details for XRCC5

Target: XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))

Alternative Name: XRCC5

Background: The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Molecular Weight: 86 kDa

UniProt: P13010

Pathways: DNA Damage Repair