CDK9 antibody (Cyclin-Dependent Kinase 9) (N-Term)

Details for Product anti-CDK9 Antibody No. ABIN97135
Antigen
Epitope
N-Term, C-Term
33
16
15
13
9
8
5
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
174
96
85
7
6
6
5
3
1
1
1
Host
Rabbit
153
21
2
Clonality
Polyclonal
Conjugate
This CDK9 antibody is un-conjugated
9
7
7
7
7
7
7
4
4
2
2
2
2
2
2
2
2
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
122
71
37
27
26
16
10
7
5
4
2
2
2
1
Options
Immunogen Multiple synthetic peptides corresponding to and domains of the protein coded by the human gene cdk9 (PITALRE).
Purification Antiserum
Antigen
Alternative Name Cdk9 (CDK9 Antibody Abstract)
Background CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2.   This kinase also was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. Tat stimulates human HIV-1 viral transcription elongation.  This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation.  Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb.   The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev.  CDK9 (PITALRE) is a ubiquitously expressed nuclear protein.
Synonyms: Cell division cycle 2 like protein kinase 4 antibody, Cell division protein kinase 9 antibody, Cyclin dependent kinase 9 antibody, PITALRE antibody, Serine threonine protein kinase PITALRE antibody, TAK antibody
Gene ID 1025, 4502747
UniProt P50750
Pathways Cell Division Cycle
Application Notes This antibody has been tested for use in ELISA, immunoprecipitation, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 43 kDa corresponding to CDK9 (PITALRE) by western blotting in the appropriate cell lysate or extract. HeLa cells may be used as a positive control.
Restrictions For Research Use only
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Cyclin-Dependent Kinase 9 (CDK9) (C-Term), (N-Term) antibody (ABIN97135) anti cdk9 antibody ( 1:1500 ) was used for Western blot analysis of 1) PC3 and 2) DU1...
Image no. 2 for anti-Cyclin-Dependent Kinase 9 (CDK9) (C-Term), (N-Term) antibody (ABIN97135) Immunocytochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The ...
Product cited in: MacLachlan, Sang, De Luca, Puri, Levrero, Giordano: "Binding of CDK9 to TRAF2." in: Journal of cellular biochemistry, Vol. 71, Issue 4, pp. 467-78, 1999 (PubMed).

de Falco, Giordano: "CDK9 (PITALRE): a multifunctional cdc2-related kinase." in: Journal of cellular physiology, Vol. 177, Issue 4, pp. 501-6, 1999 (PubMed).

Fujinaga, Cujec, Peng, Garriga, Price, Graña, Peterlin: "The ability of positive transcription elongation factor B to transactivate human immunodeficiency virus transcription depends on a functional kinase domain, cyclin T1, and Tat." in: Journal of virology, Vol. 72, Issue 9, pp. 7154-9, 1998 (PubMed).

Bagella, MacLachlan, Buono, Pisano, Giordano, De Luca: "Cloning of murine CDK9/PITALRE and its tissue-specific expression in development." in: Journal of cellular physiology, Vol. 177, Issue 2, pp. 206-13, 1998 (PubMed).

Bullrich, MacLachlan, Sang, Druck, Veronese, Allen, Chiorazzi, Koff, Heubner, Croce: "Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27Kip1, to regions involved in human cancer." in: Cancer research, Vol. 55, Issue 6, pp. 1199-205, 1995 (PubMed).

Graña, De Luca, Sang, Fu, Claudio, Rosenblatt, Morgan, Giordano: "PITALRE, a nuclear CDC2-related protein kinase that phosphorylates the retinoblastoma protein in vitro." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 9, pp. 3834-8, 1994 (PubMed).