NFkB cRel (C-Term) antibody

Details for Product No. ABIN97868
Antigen
Epitope
C-Term
47
27
10
9
9
7
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
201
50
20
6
6
5
5
4
3
3
2
1
1
Host
Rabbit
177
24
7
Clonality
Polyclonal
Conjugate
Un-conjugated
14
7
7
7
7
7
7
6
6
5
5
5
1
1
1
1
1
1
Application
Western Blotting (WB)
141
87
49
25
24
13
6
5
5
3
2
1
1
1
Options
Immunogen NFkB cRel peptide corresponding to a region near the of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Antigen
Background NFkB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells.  It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.
Synonyms: NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB
Gene ID 4790
UniProt P19838
Application Notes This product was assayed by immunoblot and found to be reactive against NFkB cRel at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat) Anti-NFkB cRel is suitable for the detection by immunoblot of human NFkB cRel.  No reaction was observed against the anologous Mouse protein.  This product was also tested in a gel supershift assay and found to be reactive against all cRel containing human NFkB complexes using 0.5 to 1.0 µl per assay.  This product was assayed by immunoblot and found to be reactive against NFkB cRel at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) Anti-NFkB cRel is suitable for the detection by immunoblot of human NFkB cRel.  No reaction was observed against the anologous Mouse protein.  This product was also tested in a gel supershift assay and found to be reactive against all cRel containing human NFkB complexes using 0.5 to 1.0 µl per assay.  This product was assayed by immunoblot and found to be reactive against NFkB cRel at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) Anti-NFkB cRel is suitable for the detection by immunoblot of human NFkB cRel.  No reaction was observed against the anologous Mouse protein.  This product was also tested in a gel supershift assay and found to be reactive against all cRel containing human NFkB complexes using 0.5 to 1.0 µl per assay. 
Restrictions For Research Use only
Format Liquid
Concentration 80 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-NFkB cRel (C-Term) antibody (ABIN97868) Western blot of HeLa cell extract. All incubations except color development were perf...