Cyclin D1 antibody (CCND1)

Details for Product anti-CCND1 Antibody No. ABIN97954
Antigen
Reactivity
Human
476
238
219
46
18
14
13
11
4
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
350
170
1
Clonality
Polyclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
31
23
15
6
6
6
6
6
6
6
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
433
168
166
105
103
76
67
40
37
25
15
9
8
8
7
3
3
2
1
1
1
Options
Immunogen Anti-Cyclin D1 was produced by repeated immunizations of full length fusion protein corresponding to the human gene sequence.
Immunogentype:Native
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Antigen
Alternative Name Cyclin D1 (CCND1 Antibody Abstract)
Background Cyclin D1 (also known as G1/S-specific cyclin D1, PRAD1 oncogene, BCL-1 oncogene, and PRAD1: parathyroid adenomatosis 1) is encoded by a gene that belongs to the highly conserved cyclin family.  Cyclins are characterized by a dramatic periodicity in protein abundance throughout the cell cycle and function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns, which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
Synonyms: G1/S-specific cyclin-D1 PRAD1 oncogene BCL-1 oncogene
Gene ID 595, 16950655
UniProt P24385
Pathways PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling
Application Notes This antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kDa in size corresponding to Cyclin D1 by western blotting in the appropriate cell lysate or extract. MCF7 may be used as a positive control. Anti-Cyclin D1is suitable for the detection by immunoblot of human, rat and mouse Cyclin D1.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Cyclin D1 (CCND1) antibody (ABIN97954) Western blot analysis is shown using Anti-Cyclin D1 antibody to detect Human Cyclin ...
Product cited in: Wetzel-Strong, Li, Klein, Nishikimi, Caron: "Epicardial-derived adrenomedullin drives cardiac hyperplasia during embryogenesis." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 243, Issue 2, pp. 243-56, 2014 (PubMed).

Kenerson, Aicher, True, Yeung: "Activated mammalian target of rapamycin pathway in the pathogenesis of tuberous sclerosis complex renal tumors." in: Cancer research, Vol. 62, Issue 20, pp. 5645-50, 2002 (PubMed).