Cyclin E1 antibody (CCNE1)

Details for Product anti-CCNE1 Antibody No. ABIN97973
Antigen
Reactivity
Human
301
75
60
4
4
4
3
Host
Rabbit
275
41
1
Clonality
Polyclonal
Conjugate
This Cyclin E1 antibody is un-conjugated
13
9
9
9
9
9
9
5
5
3
2
2
2
2
2
2
2
Application
ELISA
205
117
93
79
57
51
27
24
14
11
6
5
3
3
2
2
2
2
1
1
1
Options
Immunogen Anti-Cyclin-E Antibody was produced by repeated immunizations with a KLH conjugated peptide corresponding to a region near the carboxy terminus of human Cyclin E.
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Antigen
Alternative Name Cyclin E (CCNE1 Antibody Abstract)
Background Anti-Cyclin-E antibody detects cyclin-E. Cyclin E is a member of the cyclin family. Cyclin E binds to the G1 phase Cdk2, which is required for the transition from G1 to S phase of the cell cycle that determines cell division. The Cyclin E/CDK2 complex phosphorylates p27Kip1, tagging it for degradation, thus promoting expression of Cyclin A, allowing progression to S phase. Anti-cyclin-e Antibody is ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research.
Synonyms: CCNE antibody, CCNE1 antibody, Cyclin E1 antibody, Cyclin Es antibody, Cyclin Et antibody, CyclinE antibody, G1/S specific cyclin E antibody
Gene ID 898
UniProt P24864
Pathways Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases
Application Notes Anti-Cyclin-E antibody is suitable for the detection by immunoblot, ELISA and IP. Researchers should determine optimal titers for applications that are not stated below. Antiserum will specifically react with a 45 kDa Cyclin E protein from human tissue.
Restrictions For Research Use only
Format Liquid
Concentration 70 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Simone, Resta, Bagella, Giordano, Guanti: "Cyclin E and chromosome instability in colorectal cancer cell lines." in: Molecular pathology : MP, Vol. 55, Issue 3, pp. 200-3, 2002 (PubMed).

Claudio, De Luca, Howard, Baldi, Firpo, Koff, Paggi, Giordano: "Functional analysis of pRb2/p130 interaction with cyclins." in: Cancer research, Vol. 56, Issue 9, pp. 2003-8, 1996 (PubMed).

Koff, Giordano, Desai, Yamashita, Harper, Elledge, Nishimoto, Morgan, Franza, Roberts: "Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle." in: Science (New York, N.Y.), Vol. 257, Issue 5077, pp. 1689-94, 1992 (PubMed).

Koff, Cross, Fisher, Schumacher, Leguellec, Philippe, Roberts: "Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family." in: Cell, Vol. 66, Issue 6, pp. 1217-28, 1991 (PubMed).