Cyclin T1 antibody (CCNT1) (Internal Region)

Details for Product anti-CCNT1 Antibody No. ABIN97976
Antigen
Epitope
Internal Region
13
11
8
6
5
4
3
3
2
1
1
1
1
1
1
1
Reactivity
Human
76
35
20
4
3
3
3
3
2
2
2
1
1
1
1
Host
Rabbit
71
4
1
Clonality
Polyclonal
Conjugate
This Cyclin T1 antibody is un-conjugated
4
3
3
2
2
2
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
60
42
26
21
13
11
10
2
1
1
1
Options
Immunogen Cyclin T1 peptide corresponding to an of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Antigen
Alternative Name Cyclin T1 (CCNT1 Antibody Abstract)
Background Cyclin T1, together with the kinase CDK9, is a component of the transcription elongation factor P-TEFb which binds the human immunodeficiency virus type 1 (HIV-1) transactivator Tat. Tat stimulates human HIV-1 viral transcription elongation.  This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb.   P-TEFb facilitates transcription by phosphorylating the carboxy-terminal domain (CTD) of RNA polymerase II. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev.  Cyclin T1 is an exceptionally large cyclin and is therefore a candidate for interactions with regulatory proteins.
Synonyms: cyclin-T1 antibody, CYCT 1 antibody, cycT1 antibody, HIVE1 antibody, Human immunodeficiency virus 1 expression antibody
Gene ID 904
NCBI Accession NP_001231
UniProt O60563
Application Notes Suitable for ELISA, immunoprecipitation, immunohistochemistry, immunoblotting and other immunological methods requiring high titer and specificity.  Anti-Cyclin T1 is suitable for the detection by immunoblot of human, rat and mouse Cyclin T1.  Use paraffin embedded tissue for immunohistochemistry. HeLa cells may be used as a positive control.
Restrictions For Research Use only
Format Liquid
Concentration 75 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Cyclin T1 (CCNT1) (Internal Region) antibody (ABIN97976) Amino acids are numbered on the bottom of each protein. The cyclin box is indicated. ...