ERK2 antibody (Mitogen-Activated Protein Kinase 1) (C-Term)

Details for Product anti-MAPK1 Antibody No. ABIN98943
Antigen
Epitope
C-Term
43
25
19
11
10
9
8
7
6
5
5
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
297
135
129
26
26
15
11
9
8
8
7
6
4
2
2
2
1
1
1
Host
Rabbit
196
100
8
7
3
Clonality
Polyclonal
Conjugate
This ERK2 antibody is un-conjugated
13
8
6
4
4
3
3
3
3
3
3
3
3
3
2
2
1
Application
ELISA, Western Blotting (WB)
276
137
104
58
54
41
22
11
10
9
5
5
2
1
1
1
1
1
1
Options
Immunogen This whole rabbit antiserum was prepared by repeated immunizations with a GST fusion protein. The epitope maps near the carboxy-terminus of human p42 MAP Kinase (ERK2) protein. The epitope is identical to the corresponding sequence in mouse and differs from the rat sequence by a single, conservative amino acid substitution.
Immunogentype:Recombinant
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Antigen
Alternative Name Erk2 (MAPK1 Antibody Abstract)
Background Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK’s) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively.  When activated, ERK’s form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the up-regulation of immediate early genes.  As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli.  Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.  In vertebrates the mitogen-induced sequential activation of the kinases Raf1->Mek1->Erk2->Rsk occurs via the G-protein Ras.
Synonyms: MAP kinase 1, MAP kinase 2, p42-MAPK
Gene ID 5594, 66932916
UniProt P28482
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals
Application Notes This antiserum was tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 42 kDa in size corresponding to p42 MAP Kinase (ERK2) by western blotting in the appropriate cell lysate or extract.  p42 MAP Kinase (ERK2) is a ubiquitous protein kinase target for Ras and Raf.  The following cell lines have been assayed by immunoblot and were found to be positive for p42 MAP Kinase (ERK2) using this reagent:  U937, HeLa,  NIH-3T3, RAW 264.7, LNCaP and HEK whole cell lysates.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (C-Term) antibody (ABIN98943) Western blot using Affinity Purified anti-p42 MAP Kinase (ERK2) antibody shows detec...