Fluorescein antibody

Catalog No. ABIN99340

Product Details

Target: Fluorescein

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunomicroscopy (IM)

Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein conjugated Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.

Characteristics: Anti Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Sterility: Sterile filtered

Immunogen: Fluorescein conjugated to Goat IgG
Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

References

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Guest, Ilic, Scrable, Sell: "Survival of irradiated recipient mice after transplantation of bone marrow from young, old and "early aging" mice." in: Aging, Vol. 7, Issue 12, pp. 1212-23, (2016) (PubMed).

Miyagawa-Yamaguchi, Kotani, Honke: "Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains." in: PLoS ONE, Vol. 9, Issue 3, pp. e93054, (2015) (PubMed).

Guest, Ilic, Ma, Grant, Glinsky, Sell: "Direct and indirect contribution of bone marrow-derived cells to cancer." in: International journal of cancer, Vol. 126, Issue 10, pp. 2308-18, (2010) (PubMed).

Noga, Udomkusonsri: "Fluorescein: a rapid, sensitive, nonlethal method for detecting skin ulceration in fish." in: Veterinary pathology, Vol. 39, Issue 6, pp. 726-31, (2002) (PubMed).

Target Details

Target: Fluorescein

Target Type: Chemical

Background: Anti Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Synonyms: FITC