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|Antigen||BCL2-Antagonist/killer 1 (BAK1) Antibodies|
|Epitope||AA 22-211 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This BAK1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
Product Details anti-BAK1 AntibodyTarget Details BAK1 Application Details Handling References for anti-BAK1 antibody (ABIN3042342) Images
|Purpose||Rabbit IgG polyclonal antibody for Bcl-2 homologous antagonist/killer(BAK1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Bcl-2 homologous antagonist/killer(BAK1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: BCL2-antagonist/killer 1
Protein Name: Bcl-2 homologous antagonist/killer
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human BAK recombinant protein (Position: A22-S211). Human BAK shares 78.3 % amino acid (aa) sequence identity with mouse BAK.|
|Plasmids, Primers & others|
Target Details BAK1Product Details anti-BAK1 Antibody Application Details Handling References for anti-BAK1 antibody (ABIN3042342) Images back to top
|Alternative Name||BAK1 (BAK1 Antibody Abstract)|
BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene. The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons. By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
Synonyms: Apoptosis Regulator Bak antibody|BAK antibody|BAK like antibody|Bak NT antibody|BAK_HUMAN antibody|BAK1 antibody|Bcl 2 homologous antagonist/killer antibody|Bcl 2 like 7 protein antibody|Bcl-2 homologous antagonist/killer antibody|Bcl-2-like protein 7 antibody|BCL2 antagonist/killer 1 antibody|Bcl2 like 7 Protein antibody|Bcl2-L-7 antibody|BCL2L7 antibody|CDN1 antibody|Cell death inhibitor 1 antibody|MGC117255 antibody|MGC3887 antibody| NBak antibody|Pro apoptotic protein BAK antibody
|Pathways||Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response|
Application DetailsProduct Details anti-BAK1 Antibody Target Details BAK1 Handling References for anti-BAK1 antibody (ABIN3042342) Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-BAK1 Antibody Target Details BAK1 Application Details References for anti-BAK1 antibody (ABIN3042342) Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
References for anti-BAK1 antibody (ABIN3042342)Product Details anti-BAK1 Antibody Target Details BAK1 Application Details Handling Images back to top
|Product cited in:||
Zuo, Wu, Xu, Yang, Yan, Tan, Meng, Ying, Liu, Kang, Huang: "Minicircle-oriP-IFNγ: a novel targeted gene therapeutic system for EBV positive human nasopharyngeal carcinoma." in: PLoS ONE, Vol. 6, Issue 5, pp. e19407, 2011
Tong, Zheng, Wang, Liu, Qian: "BAK overexpression mediates p53-independent apoptosis inducing effects on human gastric cancer cells." in: BMC cancer, Vol. 4, pp. 33, 2004
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