anti-Human BAK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended BAK1 Antibody

Antigen
BCL2-Antagonist/killer 1 (BAK1) Antibodies
  • BAK
  • BAK-LIKE
  • BCL2L7
  • CDN1
  • Bak
  • N-BAK1
  • N-Bak
  • Bak1
  • bak
  • bak1
  • BCL2 antagonist/killer 1
  • BCL2-antagonist/killer 1
  • BCL2 antagonist/killer 1 L homeolog
  • BAK1
  • Bak1
  • bak1.L
Epitope
AA 22-211
62
15
9
9
5
5
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
200
77
44
6
3
3
1
1
1
Host
Rabbit
168
20
13
1
Clonality
Polyclonal
Conjugate
This BAK1 antibody is un-conjugated
7
4
4
4
4
4
4
4
4
3
3
3
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
156
87
66
28
25
19
15
13
6
5
4
4
2
Options

Available images

Catalog No. ABIN3042342
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
19.549648 ABIN2857046 IHC (p) WB Rabbit IgG N-Term Polyclonal 0
19.286304 ABIN674409 IF (p) IHC (p) WB Rabbit IgG AA 20-65 Polyclonal 2
19 ABIN4950296 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 22-211 Polyclonal 0
17.786304 ABIN615464 IHC (p) WB Rabbit AA 1-14, N-Term Polyclonal 0
14.786304 ABIN462023 IHC IHC (p) Rabbit N-Term Polyclonal 0
14.786304 ABIN615093 IHC (p) Rabbit N-Term Polyclonal 0
14.786304 ABIN461962 IF IHC IHC (p) IP WB Rabbit Polyclonal 0
13.549647 ABIN2134491 ICC IHC (p) WB Rabbit IgG Middle Region Polyclonal 0
13 ABIN573998 IHC IHC (p) WB Rabbit AA 1-14 Polyclonal 0
11.5 ABIN543401 IHC (p) ELISA WB Rabbit Polyclonal 3
11.5 ABIN537485 IHC (p) IP Rabbit Polyclonal 1
11.233593 ABIN5949407 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG AA 1-100 SU32-07 1
10 ABIN1732214 IHC (p) WB Rabbit IgG AA 1-14 Polyclonal 0
7 ABIN356810 EIA IHC (p) WB Rabbit Ig Fraction BH3 Domain, N-Term Polyclonal 5
7 ABIN1494304 IHC IHC (p) WB Rabbit IgG N-Term Polyclonal 0
7 ABIN957151 IHC IHC (fro) IHC (p) IP WB Rabbit AA 16-36 Polyclonal 0
7 ABIN265324 IF IHC (p) WB Rabbit Polyclonal 0
7 ABIN1732215 IHC (p) Rabbit IgG N-Term Polyclonal 0
7 ABIN5648908 ELISA IHC (p) WB Rabbit Polyclonal 0
5.2335935 ABIN4282996 IHC IHC (p) WB Rabbit Polyclonal 1

General

Antigen BCL2-Antagonist/killer 1 (BAK1) Antibodies
Epitope AA 22-211
(62), (15), (9), (9), (5), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(200), (77), (44), (6), (3), (3), (1), (1), (1)
Host Rabbit
(168), (20), (13), (1)
Clonality
Polyclonal
Conjugate This BAK1 antibody is un-conjugated
(7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(156), (87), (66), (28), (25), (19), (15), (13), (6), (5), (4), (4), (2)
Pubmed 2 references available
Supplier

Product Details anti-BAK1 Antibody

Target Details BAK1 Application Details Handling References for anti-BAK1 antibody (ABIN3042342) Images
Purpose Rabbit IgG polyclonal antibody for Bcl-2 homologous antagonist/killer(BAK1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Bcl-2 homologous antagonist/killer(BAK1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: BCL2-antagonist/killer 1
Protein Name: Bcl-2 homologous antagonist/killer
Purification Immunogen affinity purified.
Immunogen E.coli-derived human BAK recombinant protein (Position: A22-S211). Human BAK shares 78.3 % amino acid (aa) sequence identity with mouse BAK.
Isotype IgG
Plasmids, Primers & others

Target Details BAK1

Product Details anti-BAK1 Antibody Application Details Handling References for anti-BAK1 antibody (ABIN3042342) Images back to top
Antigen
Alternative Name BAK1 (BAK1 Antibody Abstract)
Background BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene. The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons. By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Synonyms: Apoptosis Regulator Bak antibody|BAK antibody|BAK like antibody|Bak NT antibody|BAK_HUMAN antibody|BAK1 antibody|Bcl 2 homologous antagonist/killer antibody|Bcl 2 like 7 protein antibody|Bcl-2 homologous antagonist/killer antibody|Bcl-2-like protein 7 antibody|BCL2 antagonist/killer 1 antibody|Bcl2 like 7 Protein antibody|Bcl2-L-7 antibody|BCL2L7 antibody|CDN1 antibody|Cell death inhibitor 1 antibody|MGC117255 antibody|MGC3887 antibody| NBak antibody|Pro apoptotic protein BAK antibody
Gene ID 578
UniProt Q16611
Pathways Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response

Application Details

Product Details anti-BAK1 Antibody Target Details BAK1 Handling References for anti-BAK1 antibody (ABIN3042342) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-BAK1 Antibody Target Details BAK1 Application Details References for anti-BAK1 antibody (ABIN3042342) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-BAK1 antibody (ABIN3042342)

Product Details anti-BAK1 Antibody Target Details BAK1 Application Details Handling Images back to top
Product cited in:

Zuo, Wu, Xu, Yang, Yan, Tan, Meng, Ying, Liu, Kang, Huang: "Minicircle-oriP-IFNγ: a novel targeted gene therapeutic system for EBV positive human nasopharyngeal carcinoma." in: PLoS ONE, Vol. 6, Issue 5, pp. e19407, 2011

Tong, Zheng, Wang, Liu, Qian: "BAK overexpression mediates p53-independent apoptosis inducing effects on human gastric cancer cells." in: BMC cancer, Vol. 4, pp. 33, 2004

Images

Product Details anti-BAK1 Antibody Target Details BAK1 Application Details Handling References for anti-BAK1 antibody (ABIN3042342) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BCL2-Antagonist/killer 1 (BAK1) (AA 22-211) antibody (ABIN3042342) Anti- BAK Picoband antibody, IHC(P) IHC(P): Rat Skeletal Muscle Tissue
Western Blotting (WB) image for anti-BCL2-Antagonist/killer 1 (BAK1) (AA 22-211) antibody (ABIN3042342) Anti- BAK Picoband antibody, Western blotting All lanes: Anti BAK at 0.5ug/ml Lane 1...
Immunohistochemistry (IHC) image for anti-BCL2-Antagonist/killer 1 (BAK1) (AA 22-211) antibody (ABIN3042342) Anti- BAK Picoband antibody, IHC(P) IHC(P): Mouse Skeletal Muscle Tissue
Immunohistochemistry (IHC) image for anti-BCL2-Antagonist/killer 1 (BAK1) (AA 22-211) antibody (ABIN3042342) Anti- BAK Picoband antibody, IHC(P) IHC(P): Human Intestinal Cancer Tissue
Western Blotting (WB) image for anti-BCL2-Antagonist/killer 1 (BAK1) (AA 22-211) antibody (ABIN3042342) Western blot analysis of BAK using anti-BAK antibody . Electrophoresis was performed...
Immunohistochemistry (IHC) image for anti-BCL2-Antagonist/killer 1 (BAK1) (AA 22-211) antibody (ABIN3042342) IHC analysis of BAK using anti-BAK antibody . BAK was detected in frozen section of ...
Immunohistochemistry (IHC) image for anti-BCL2-Antagonist/killer 1 (BAK1) (AA 22-211) antibody (ABIN3042342) IHC analysis of BAK using anti-BAK antibody . BAK was detected in frozen section of ...
Immunohistochemistry (IHC) image for anti-BCL2-Antagonist/killer 1 (BAK1) (AA 22-211) antibody (ABIN3042342) IHC analysis of BAK using anti-BAK antibody . BAK was detected in immunocytochemical...
Immunohistochemistry (IHC) image for anti-BCL2-Antagonist/killer 1 (BAK1) (AA 22-211) antibody (ABIN3042342) IHC analysis of BAK using anti-BAK antibody . BAK was detected in immunocytochemical...
Immunohistochemistry (IHC) image for anti-BCL2-Antagonist/killer 1 (BAK1) (AA 22-211) antibody (ABIN3042342) IHC analysis of BAK using anti-BAK antibody . BAK was detected in frozen section of ...