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|Antigen||BH3 Interacting Domain Death Agonist (BID) Antibodies|
|Conjugate||This BID antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
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Product Details anti-BID AntibodyTarget Details BID Application Details Handling References for anti-BID antibody (ABIN358089) Images
|Specificity||This antibody detects BID pSer65.|
|Purification||Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S65 of human Bid.|
Target Details BIDProduct Details anti-BID Antibody Application Details Handling References for anti-BID antibody (ABIN358089) Images back to top
|Alternative Name||BID (BID Antibody Abstract)|
|Background||Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.Synonyms: BH3-interacting domain death agonist, p22 BID|
|Molecular Weight||26836 Da|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-BID Antibody Target Details BID Handling References for anti-BID antibody (ABIN358089) Images back to top
ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BID Antibody Target Details BID Application Details References for anti-BID antibody (ABIN358089) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-BID antibody (ABIN358089)Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
Liu, Durrant, Yang, He, Whitby, Myszka, Lee: "The interaction between tBid and cardiolipin or monolysocardiolipin." in: Biochemical and biophysical research communications, Vol. 330, Issue 3, pp. 865-70, 2005
Broaddus, Dansen, Abayasiriwardana, Wilson, Finch, Swigart, Hunt, Evan: "Bid mediates apoptotic synergy between tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and DNA damage." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12486-93, 2005
Weng, Li, Xu, Shi, Tang: "Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10491-500, 2005
Gong, Choi, Franzin, Zhai, Reed, Marassi: "Conformation of membrane-associated proapoptotic tBid." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28954-60, 2004
García-Sáez, Mingarro, Pérez-Payá, Salgado: "Membrane-insertion fragments of Bcl-xL, Bax, and Bid." in: Biochemistry, Vol. 43, Issue 34, pp. 10930-43, 2004
ImagesProduct Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID antibody (ABIN358089) back to top