anti-Human BID antibody for Western Blotting

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • BH3 interacting domain death agonist
  • BH3 interacting domain death agonist S homeolog
  • BID
  • Bid
  • bida
  • bid.S
  • bid
Reactivity
Human
323
174
74
9
5
3
2
Host
Rabbit
298
96
20
9
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
21
19
14
9
8
7
4
4
3
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
356
192
128
90
49
41
35
26
14
11
11
6
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN222962
$ 245.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2573495 ABIN2788659 IF WB Rabbit C-Term Log in to see Polyclonal 0
3.2573495 ABIN4284616 WB Rabbit AA 120-180 Log in to see Polyclonal 1
3.2573495 ABIN2705594 WB Rabbit Center Log in to see Polyclonal 0
3.2573495 ABIN389523 IHC (p) WB Rabbit Ig Fraction pSer65 Log in to see Polyclonal 5
3.2573495 ABIN3042321 ICC IHC (p) WB Rabbit IgG AA 1-195 Log in to see Polyclonal 0
3.2573495 ABIN2957879 IC IF IHC WB Rabbit Log in to see Polyclonal 0
3.2573495 ABIN1532322 IHC ELISA WB Rabbit IgG AA 44-93 Log in to see Polyclonal 1
3.2573495 ABIN968577 IF WB Mouse IgG1 AA 50-62 Log in to see 7-Bid 5
3.2573495 ABIN388099 IHC (p) WB Rabbit Ig Fraction AA 68-103, BH3 Domain Log in to see Polyclonal 3
3.2573495 ABIN4284618 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 1
3.2573495 ABIN4284621 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 3
3.2573495 ABIN968979 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 3C5 2
3.2573495 ABIN4284615 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 4
3.2573495 ABIN968686 IF WB Mouse IgG1 AA 46-168 Log in to see 40-Bid 3
3.2573495 ABIN336094 EIA WB Mouse IgG1 AA 1-195, N-Term Log in to see 4D3 0
3.2573495 ABIN449668 SDS ELISA WB Mouse IgG1 kappa AA 1-195 Log in to see 4D3 0
3.2573495 ABIN250381 ELISA WB Goat C-Term, all Isoforms Log in to see Polyclonal 1
3.2573495 ABIN153045 IHC (p) IHC WB Rabbit AA 60-120 Log in to see Polyclonal 2
3.2573495 ABIN1996891 ELISA ELISA (Detection) IP WB Mouse IgG2a AA 1-195 Log in to see 3C4C2 0
3.2573495 ABIN1996893 CC ELISA FACS IF IF/ICC WB Mouse IgG1 AA 1-195 Log in to see 10 0

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Reactivity Human
(323), (174), (74), (9), (5), (3), (2)
Host Rabbit
(298), (96), (20), (9)
Clonality
Polyclonal
Conjugate This BID antibody is un-conjugated
(21), (19), (14), (9), (8), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(356), (192), (128), (90), (49), (41), (35), (26), (14), (11), (11), (6), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-BID Antibody

Target Details BID Application Details Handling References for anti-BID antibody (ABIN222962) Images
Blocking Peptide Blocking peptide for this product available: ABIN412616
Specificity The antibody detects 22 kDa human Bid .
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 37 of human Bid
Isotype IgG

Target Details BID

Product Details anti-BID Antibody Application Details Handling References for anti-BID antibody (ABIN222962) Images back to top
Antigen
Alternative Name Bid (BID Antibody Abstract)
Background Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed throμgh Fas.
Synonyms: FP497 , MGC42355, MGC15319
Gene ID 637
UniProt P55957
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BID Antibody Target Details BID Handling References for anti-BID antibody (ABIN222962) Images back to top
Application Notes Western blot analysis (0.5-4 μg/mL), immunoprecipitation (5-10 μg/mL), and Immunohistochemistry (20-40 μg/mL). However, the optimal conditions should be determined individually. The antibody detects 22 kDa human Bid
Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details References for anti-BID antibody (ABIN222962) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit anti-Bid polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-BID antibody (ABIN222962)

Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
Product cited in:

Liang, Yang, Huang, Lin, Chou, Chen, Pan: "Antegrade retrieval of ureteral stents through an 8-French percutaneous nephrostomy route." in: AJR. American journal of roentgenology, Vol. 191, Issue 5, pp. 1530-5, 2008

Chen, Huang, Wang: "Deficiency of Bim in dendritic cells contributes to overactivation of lymphocytes and autoimmunity." in: Blood, Vol. 109, Issue 10, pp. 4360-7, 2007

Huang, Masselli, Frisch, Hunton, Jiang, Wang: "Blockade of tumor necrosis factor-induced Bid cleavage by caspase-resistant Rb." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29401-13, 2007

Ju, Valencia, Pang, Ke, Gao, Dong, Liu: "Proteome-wide identification of family member-specific natural substrate repertoire of caspases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 36, pp. 14294-9, 2007

Kulik, Carson, Vomastek, Overman, Gooch, Srinivasula, Alnemri, Nunez, Weber: "Tumor necrosis factor alpha induces BID cleavage and bypasses antiapoptotic signals in prostate cancer LNCaP cells." in: Cancer research, Vol. 61, Issue 6, pp. 2713-9, 2001

Wang, Wieckowski, Goldstein, Gastman, Rabinovitz, Gambotto, Li, Fang, Yin, Rabinowich: "Resistance to granzyme B-mediated cytochrome c release in Bak-deficient cells." in: The Journal of experimental medicine, Vol. 194, Issue 9, pp. 1325-37, 2001

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID antibody (ABIN222962) back to top
Supplier Images
Western Blotting (WB) image for anti-BH3 Interacting Domain Death Agonist (BID) antibody (ABIN222962) Western blot analysis of Bid expression in Jurkat cell lysate.