anti-Human Caspase 2 antibody for Western Blotting

Recommended Caspase 2 Antibody (supplied by: Log in to see )

Antigen
Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) Antibodies
  • xCaspase-2
  • Caspase-2
  • ICH-1
  • Nedd2
  • CASP-2
  • ICH1
  • NEDD-2
  • NEDD2
  • PPP1R57
  • ICH-1L/1S
  • ICH1L1S
  • caspase 2
  • caspase 2 L homeolog
  • caspase-2
  • CASP2
  • casp2.L
  • CpipJ_CPIJ008254
  • Casp2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
230
102
75
12
10
2
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
182
44
15
Clonality
Polyclonal
Conjugate
This Caspase 2 antibody is un-conjugated
8
8
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
201
108
73
36
20
17
14
14
13
6
4
4
4
Options
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Catalog No. ABIN222882
$ 255.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1493003 ABIN2787902 WB Rabbit Middle Region Log in to see Polyclonal 0
3.1493003 ABIN3043696 WB Rabbit IgG AA 417-439, C-Term Log in to see Polyclonal 27
3.1493003 ABIN3043468 WB Rabbit IgG AA 378-409, C-Term Log in to see Polyclonal 0
3.1493003 ABIN2705678 IHC WB Rabbit Center Log in to see Polyclonal 0
3.1493003 ABIN1536102 ELISA WB Rabbit IgG AA 276-325, Cleaved-Thr325 Log in to see Polyclonal 2
3.1493003 ABIN1536101 ELISA WB Rabbit IgG AA 151-200, Cleaved-Gly170 Log in to see Polyclonal 2
3.1493003 ABIN1532667 IHC ELISA WB Rabbit IgG AA 123-172 Log in to see Polyclonal 1
3.1493003 ABIN388122 FACS IHC (p) WB Rabbit Ig Fraction AA 198-226, Center Log in to see Polyclonal 3
3.1493003 ABIN180742 EIA WB Mouse IgG2b AA 32-169 Log in to see 1C10 0
3.1493003 ABIN316066 ELISA WB Mouse IgG2b AA 32-169 Log in to see 1C10 0
3.1493003 ABIN4288070 WB Rabbit IgG Center Log in to see Polyclonal 0
3.1493003 ABIN4288071 ELISA ICC IF WB Rabbit N-Term Log in to see Polyclonal 0
3.1493003 ABIN252631 WB Mouse IgM kappa Log in to see 18E809-3 0
3.1493003 ABIN3183666 ELISA IHC WB Rabbit IgG Ser197 Log in to see Polyclonal 0
3.1493003 ABIN1077908 ICC IHC WB Rabbit IgG AA 334-452 Log in to see Polyclonal 0
3.1493003 ABIN1539971 WB Mouse IgG1 AA 432-461 Log in to see 550CT8-5-2 0
3.1493003 ABIN1031296 ICC ELISA WB Rabbit N-Term Log in to see Polyclonal 0
3.1493003 ABIN4903135 FACS IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1493003 ABIN3030295 FACS IHC ELISA WB Rabbit Ig Fraction AA 198-226 Log in to see Polyclonal 0
3.1493003 ABIN2561574 IHC WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(230), (102), (75), (12), (10), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(182), (44), (15)
Clonality
Polyclonal
Conjugate This Caspase 2 antibody is un-conjugated
(8), (8), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(201), (108), (73), (36), (20), (17), (14), (14), (13), (6), (4), (4), (4)
Pubmed 2 references available
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Product Details anti-Caspase 2 Antibody

Target Details Caspase 2 Application Details Handling References for anti-Caspase 2 antibody (ABIN222882) Images
Blocking Peptide Blocking peptide for this product available: ABIN412649
Specificity Detects the full length and small subunit of caspase-2.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 417 of mouse caspase-2
Isotype IgG
Plasmids, Primers & others

Target Details Caspase 2

Product Details anti-Caspase 2 Antibody Application Details Handling References for anti-Caspase 2 antibody (ABIN222882) Images back to top
Antigen
Alternative Name Caspase-2 (CASP2 Antibody Abstract)
Background Caspases play a key role in apoptosis. Similar as other caspases, caspase-2 is also synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis. Caspase-2 mRNA is alternatively spliced. The larger mRNA species encoding a product of 435 amino acids is known as ICH-1L. The smaller mRNA species encoding a protein of 312 amino acids is named ICH-1S. Over expression of ICH-1L induces apoptosis, while over expression of ICH-1S suppresses Rat-1 cell death induced by serum deprivation.
Synonyms: CASP-2, NEDD-2,NEDD2, ICH1, ICH-1L/1S, ICH-1L, EC 3.4.22.55
Gene ID 12366
Pathways Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 2 Antibody Target Details Caspase 2 Handling References for anti-Caspase 2 antibody (ABIN222882) Images back to top
Application Notes Western blotting (1-2 μg/mL), and Immunohistochemistry (5 μg/mL). However, the optimal conditions should be determined individually. Detects the full length and small subunit of caspase-2. In human samples, it also detects a 30 kDa intermeadiate fragment
Comment

Suggested positive control:
Western Blot: Jurkat cell lysate, treated and untreated. IHC: Placenta tissue.

Restrictions For Research Use only

Handling

Product Details anti-Caspase 2 Antibody Target Details Caspase 2 Application Details References for anti-Caspase 2 antibody (ABIN222882) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-caspase-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-Caspase 2 antibody (ABIN222882)

Product Details anti-Caspase 2 Antibody Target Details Caspase 2 Application Details Handling Images back to top
Product cited in:

Zhu, Sun, Okano, Chen, Zhang, Maeda, Palczewski: "Sponge transgenic mouse model reveals important roles for the microRNA-183 (miR-183)/96/182 cluster in postmitotic photoreceptors of the retina." in: The Journal of biological chemistry, Vol. 286, Issue 36, pp. 31749-60, 2011

Basu, Adkins, Basu: "Down-regulation of caspase-2 by rottlerin via protein kinase C-delta-independent pathway." in: Cancer research, Vol. 68, Issue 8, pp. 2795-802, 2008

Images

Product Details anti-Caspase 2 Antibody Target Details Caspase 2 Application Details Handling References for anti-Caspase 2 antibody (ABIN222882) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) antibody (ABIN222882) Western blot analysis of caspase-2 in untreated (Lane and treated (Lane cell lysate.