anti-Human Caspase 3 antibody for Immunocytochemistry

Recommended Caspase 3 Antibody (supplied by: Log in to see )

Antigen
Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antibodies
  • CPP32
  • CPP32B
  • SCA-1
  • A830040C14Rik
  • AC-3
  • Apopain
  • CC3
  • Caspase-3
  • Lice
  • Yama
  • mldy
  • xcpp32
  • casp3
  • zgc:100890
  • CASP-3
  • caspase-3
  • caspase 3
  • caspase 3 S homeolog
  • caspase 3, apoptosis-related cysteine peptidase a
  • caspase 3, apoptosis-related cysteine peptidase
  • CASP3
  • Casp3
  • casp3.S
  • casp3a
Epitope
AA 220-236, C-Term
41
30
29
28
25
20
18
16
15
15
12
12
11
11
10
10
8
7
6
6
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
587
327
273
45
44
34
29
25
22
19
17
16
15
11
8
7
6
4
2
2
Host
Rabbit
500
146
19
4
2
Clonality
Polyclonal
Conjugate
This Caspase 3 antibody is un-conjugated
31
26
19
11
10
9
8
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
516
279
208
156
77
72
63
58
47
29
28
23
13
9
6
5
3
3
2
2
2
2
2
2
1
1
1
1
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Catalog No. ABIN3044357
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
42.53808 ABIN252651 CyTOF EM FACS ICC IF IHC IHC (fro) IHC (p) SimWes WB Mouse IgG1 kappa Log in to see 31A1067 114
28 ABIN6260481 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 7
26.5 ABIN6269461 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 12
23.5 ABIN6254994 ELISA ICC IF IHC WB Rabbit IgG pSer150 Log in to see Polyclonal 1
22.08413 ABIN5542609 ELISA FACS ICC IHC WB Mouse IgG1 AA 29-175 Log in to see 3D4D10 0
22.08413 ABIN5542610 ELISA FACS ICC IHC WB Mouse IgG1 AA 29-175 Log in to see 3D4D7 0
19 ABIN1980380 ICC IHC WB Rabbit IgG AA 29-175 Log in to see Polyclonal 0
16 ABIN6260474 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
14.368527 ABIN1742483 ICC Rabbit AA 170-175 Log in to see Polyclonal 0
13.08413 ABIN5619059 ICC IF IHC WB Rabbit Center Log in to see Polyclonal 0
11.5 ABIN1077909 ICC IHC WB Rabbit IgG AA 183-277 Log in to see Polyclonal 1
11.038079 ABIN4288099 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
10 ABIN3030297 ICC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
7 ABIN6271892 ELISA ICC IF WB Rabbit IgG pSer26 Log in to see Polyclonal 0
7 ABIN6266325 ELISA ICC IF IP WB Rabbit IgG Log in to see Polyclonal 0
6.5380793 ABIN4288120 ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see 31A1067 0
5.0380793 ABIN4288091 ICC IF IHC (p) IHC Rabbit IgG Log in to see Polyclonal 13
4 ABIN5662078 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see 0
1 ABIN470500 ICC FACS IF ELISA Alexa Fluor 488 Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN219228 ELISA FACS ICC IF IHC IHC (fro) WB Rabbit IgG N-Term Log in to see Polyclonal 0

General

Antigen Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antibodies
Epitope AA 220-236, C-Term
(41), (30), (29), (28), (25), (20), (18), (16), (15), (15), (12), (12), (11), (11), (10), (10), (8), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(587), (327), (273), (45), (44), (34), (29), (25), (22), (19), (17), (16), (15), (11), (8), (7), (6), (4), (2), (2)
Host Rabbit
(500), (146), (19), (4), (2)
Clonality
Polyclonal
Conjugate This Caspase 3 antibody is un-conjugated
(31), (26), (19), (11), (10), (9), (8), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(516), (279), (208), (156), (77), (72), (63), (58), (47), (29), (28), (23), (13), (9), (6), (5), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 94 references available
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Product Details anti-Caspase 3 Antibody

Target Details Caspase 3 Application Details Handling References for anti-Caspase 3 antibody (ABIN3044357) Images
Purpose Rabbit IgG polyclonal antibody for Caspase-3(CASP3) detection. Tested with WB, IHC-P, ICC in Human.
Sequence CAMLKQYADK LEFMHIL
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Caspase-3(CASP3) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: caspase 3, apoptosis-related cysteine peptidase
Protein Name: Caspase-3(CASP-3)
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-3(P10)(220-236aa CAMLKQYADKLEFMHIL).
Isotype IgG
Plasmids, Primers & others

Target Details Caspase 3

Product Details anti-Caspase 3 Antibody Application Details Handling References for anti-Caspase 3 antibody (ABIN3044357) Images back to top
Antigen
Alternative Name CASP3 (CASP3 Antibody Abstract)
Background Caspase 3 is a caspase protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located at 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease(caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7, and 9, and the protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.

Synonyms: A830040C14Rik antibody|Apopain antibody|Apopain precursor antibody|CASP 3 antibody|CASP-3 antibody|CASP3 antibody|Casp3a antibody|Caspase 3 antibody|Caspase 3 apoptosis related cysteine protease antibody|Caspase 3 p12 subunit antibody|Caspase 3, apoptosis-related cysteine peptidase antibody|Caspase 3, apoptosis-related cysteine protease antibody|Caspase 3, apoptosis-related cysteine protease a antibody|Caspase-3 subunit p12 antibody|Caspase3 antibody|CC3 antibody|CPP 32 antibody|CPP-32 antibody|CPP32 antibody|CPP32B antibody|Cysteine protease CPP32 antibody|EC 3.4.22.56 antibody|Human cysteine protease CPP32 isoform alpha mRNA complete cds antibody|ICE3 antibody|LICE antibody|mldy antibody|OTTHUMP00000165052 antibody|OTTHUMP00000165053 antibody|OTTHUMP00000165054 antibody|PARP cleavage protease antibody|Procaspase3 antibody|Protein Yama antibody|SCA 1 antibody|SCA-1 antibody|SCA1 antibody|SREBP cleavage activity 1 antibody|Yama antibody|Yama protein antibody
UniProt P42574
Pathways Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation

Application Details

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Handling References for anti-Caspase 3 antibody (ABIN3044357) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details References for anti-Caspase 3 antibody (ABIN3044357) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

References for anti-Caspase 3 antibody (ABIN3044357)

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details Handling Images back to top
Product cited in:

Bai, Yang, Luo: "Effects of 5-hydroxy-4'-nitro-7-propionyloxy-genistein on inhibiting proliferation and invasion via activating reactive oxygen species in human ovarian cancer A2780/DDP cells." in: Oncology letters, Vol. 15, Issue 4, pp. 5227-5235, 2018

Sun, Cui, Wang, Guo, Zhang: "Tumor necrosis factor-related apoptosis inducing ligand overexpression and Taxol treatment suppresses the growth of cervical cancer cells in vitro and in vivo." in: Oncology letters, Vol. 15, Issue 4, pp. 5744-5750, 2018

Yang, Shi, Soomro, Hu, Du, She: "Hepatitis E Virus Induces Hepatocyte Apoptosis via Mitochondrial Pathway in Mongolian Gerbils." in: Frontiers in microbiology, Vol. 9, pp. 460, 2018

Qi, Xue, Lv, Sun, Du, Cai, Li, Gu, Wang: "Ginkgolic acids induce HepG2 cell death via a combination of apoptosis, autophagy and the mitochondrial pathway." in: Oncology letters, Vol. 15, Issue 5, pp. 6400-6408, 2018

Xu, Xie, Wang, Wang, Luo, Zhou, Wang, Huang, Tan, Jin, Qi, Tang, Chen, Du, Zhao, Liang, Chen: "A novel fibroblast growth factor receptor 1 inhibitor protects against cartilage degradation in a murine model of osteoarthritis." in: Scientific reports, Vol. 6, pp. 24042, 2017

Hu, Sun, Zhang, Zhang, Hu: "Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway." in: Bioscience reports, Vol. 36, Issue 3, 2017

Zhou, He, Wang, Xie, Liu, Liu, Song, Ma: "Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir." in: International journal of molecular sciences, Vol. 17, Issue 6, 2017

Xu, Tan, Jiang, Chen, Lai, Zhang, Liang: "Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure." in: Chinese medicine, Vol. 12, pp. 10, 2017

Zheng, Chen, Zhu, Li, Deng, Wang, Zhang, Chen: "Liraglutide prevents fast weight gain and β-cell dysfunction in male catch-up growth rats." in: Experimental biology and medicine (Maywood, N.J.), Vol. 240, Issue 9, pp. 1165-76, 2016

Han, Liu, Li, Tian, Han, Wang, Fu, Guo, Zhu, Du, Tian: "HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142387, 2016

Ji, Geng, Zhou, Wei, Chen: "Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells." in: Neural regeneration research, Vol. 11, Issue 12, pp. 1962-1968, 2016

Kan, Zhou, Jin, Yang: "Effects of PDTC on NF-?B expression and apoptosis in rats with severe acute pancreatitis-associated lung injury." in: International journal of clinical and experimental medicine, Vol. 8, Issue 3, pp. 3258-70, 2015

Xu, Liu, Zhang, Wang, Wang, Yang, Huo, Sun: "Apoptosis-related protein expression in rabbits with blast brain injury following early hyperbaric oxygen therapy." in: Neural regeneration research, Vol. 7, Issue 17, pp. 1318-24, 2015

Ding, Wang, Zhao, Sun, Zhai: "Protective Effects of Baicalin on A?????-Induced Learning and Memory Deficit, Oxidative Stress, and Apoptosis in Rat." in: Cellular and molecular neurobiology, Vol. 35, Issue 5, pp. 623-32, 2015

Yang, Li, Liu, Shi, Zhang: "Inhibitory effect of tetramethylpyrazine preconditioning on overload training-induced myocardial apoptosis in rats." in: Chinese journal of integrative medicine, Vol. 21, Issue 6, pp. 423-30, 2015

Li, Wang, Wang: "Radioprotective activity of neutral polysaccharides isolated from the fruiting bodies of Hohenbuehelia serotina." in: Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB), Vol. 31, Issue 4, pp. 352-9, 2015

Lu, Yuan, Ou, Cai, Wang, Sun, Zhang: "Autophagy and apoptosis during adult adipose-derived stromal cells differentiation into neuron-like cells in vitro." in: Neural regeneration research, Vol. 7, Issue 16, pp. 1205-12, 2015

Zhan, Hu, Yi, An, Huang: "Inhibitory activity of apogossypol in human prostate cancer in vitro and in vivo." in: Molecular medicine reports, Vol. 11, Issue 6, pp. 4142-8, 2015

Peng, Chen, Chen, Fang, Cui, Zuo, Deng, Chen, Geng, Lai: "Aflatoxin B1 affects apoptosis and expression of Bax, Bcl-2, and Caspase-3 in thymus and bursa of fabricius in broiler chickens." in: Environmental toxicology, 2015

Chen, Peng, Rowat, Gao, Zhang, Wang, Zhang, Wang, Qu: "The effect of concentration and duration of normobaric oxygen in reducing caspase-3 and -9 expression in a rat-model of focal cerebral ischaemia." in: Brain research, Vol. 1618, pp. 205-11, 2015

Images

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details Handling References for anti-Caspase 3 antibody (ABIN3044357) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 220-236), (C-Term) antibody (ABIN3044357) Anti-Caspase-3(P10), IHC(P) IHC(P): Human Intestinal Cancer Tissue
Immunohistochemistry (IHC) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 220-236), (C-Term) antibody (ABIN3044357) Anti-Caspase-3(P10) antibody, IHC(P) IHC(P): Rat Intestine Tissue
Immunohistochemistry (IHC) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 220-236), (C-Term) antibody (ABIN3044357) Anti-Caspase-3(P10), IHC(P) IHC(P): Human Lung Cancer Tissue
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 220-236), (C-Term) antibody (ABIN3044357) Anti-Caspase-3(P10), Western blotting Lane 1: HELA Cell Lysate Lane 2: SMMC Cell Lysate
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 220-236), (C-Term) antibody (ABIN3044357) Anti-Caspase-3(P10) antibody, Western blotting Lane 1: Rat Liver Tissue Lysate Lane ...