anti-Human Caspase 3 antibody for Immunohistochemistry

Recommended Caspase 3 Antibody (supplied by: Log in to see )

Antigen
Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antibodies
  • CPP32
  • CPP32B
  • SCA-1
  • A830040C14Rik
  • AC-3
  • Apopain
  • CC3
  • Caspase-3
  • Lice
  • Yama
  • mldy
  • xcpp32
  • CASP-3
  • caspase-3
  • caspase 3
  • caspase 3 S homeolog
  • caspase 3, apoptosis-related cysteine peptidase
  • CASP3
  • Casp3
  • casp3.S
Epitope
Cleavage Site, N-Term, active
41
33
30
28
24
20
18
16
16
16
13
11
11
11
11
10
7
7
6
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
634
352
299
46
45
34
30
25
23
20
18
16
15
11
7
7
6
4
2
2
Host
Rabbit
508
185
19
4
2
Clonality
Polyclonal
Biological Activity
Active
Conjugate
This Caspase 3 antibody is un-conjugated
33
28
21
15
13
10
9
6
6
5
5
5
5
5
5
5
5
4
3
3
3
2
2
2
2
2
2
2
2
2
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
556
310
245
163
105
77
66
58
51
29
29
23
13
9
6
5
4
3
2
2
2
2
2
2
1
1
1
1
Options
Supplier
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Catalog No. ABIN222873
$ 415.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.537891 ABIN3188045 IHC WB Mouse IgG1 Log in to see 5E1 0
8.537891 ABIN2774596 IHC WB Rabbit N-Term Log in to see Polyclonal 2
8.537891 ABIN2705682 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
8.537891 ABIN1531535 IHC ELISA WB Rabbit IgG AA 116-165, pSer150 Log in to see Polyclonal 0
8.537891 ABIN1536080 IHC ELISA WB Rabbit IgG AA 126-175, Cleaved-Asp175 Log in to see Polyclonal 0
8.537891 ABIN1536079 IHC ELISA Rabbit IgG AA 126-175, Cleaved-Asp175 Log in to see Polyclonal 0
8.537891 ABIN1536081 IHC ELISA WB Rabbit IgG AA 157-206, Cleaved-Asp175 Log in to see Polyclonal 0
8.537891 ABIN1871461 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
8.537891 ABIN1871462 IF IHC IP WB Rabbit IgG Log in to see Polyclonal 0
8.537891 ABIN1077909 ICC IHC WB Rabbit IgG AA 183-277 Log in to see Polyclonal 1
8.537891 ABIN218358 IHC IHC (p) IP WB Rabbit IgG N-Term Log in to see Polyclonal 0
8.537891 ABIN305732 IHC IHC (p) WB Mouse IgG Log in to see 31A1067  0
8.537891 ABIN364239 IHC IHC (p) IP WB Rabbit IgG Log in to see Polyclonal 0
8.537891 ABIN604792 IHC IHC (p) Rabbit IgG Internal Region Log in to see Polyclonal 0
8.537891 ABIN1980380 ICC IHC WB Rabbit IgG AA 29-175 Log in to see Polyclonal 0
8.537891 ABIN3030296 FACS IHC ELISA WB Rabbit Ig Fraction AA 149-179 Log in to see Polyclonal 0
8.537891 ABIN1871464 IHC WB Rabbit IgG Log in to see Polyclonal 0
8.537891 ABIN2852719 IHC WB Rabbit Log in to see Polyclonal 0
8.537891 ABIN2470477 IHC WB Mouse IgG full length Log in to see 31A1067 0
8.537891 ABIN2852718 IHC WB Mouse Log in to see 0

General

Antigen Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antibodies
Epitope Cleavage Site, N-Term, active
(41), (33), (30), (28), (24), (20), (18), (16), (16), (16), (13), (11), (11), (11), (11), (10), (7), (7), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(634), (352), (299), (46), (45), (34), (30), (25), (23), (20), (18), (16), (15), (11), (7), (7), (6), (4), (2), (2)
Host Rabbit
(508), (185), (19), (4), (2)
Clonality
Polyclonal
Conjugate This Caspase 3 antibody is un-conjugated
(33), (28), (21), (15), (13), (10), (9), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(556), (310), (245), (163), (105), (77), (66), (58), (51), (29), (29), (23), (13), (9), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 24 references available
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Product Details anti-Caspase 3 Antibody

Target Details Caspase 3 Application Details Handling References for anti-Caspase 3 antibody (ABIN222873) Images
Blocking Peptide Blocking peptide for this product available: ABIN412650
Specificity Preferentially recognizes active human/mouse/rat caspase-3 (p20 subunits) and does not detect the precursor form
Purification Affinity purified
Immunogen Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
Isotype IgG
Plasmids, Primers & others

Target Details Caspase 3

Product Details anti-Caspase 3 Antibody Application Details Handling References for anti-Caspase 3 antibody (ABIN222873) Images back to top
Antigen
Alternative Name Caspase-3 (CASP3 Antibody Abstract)
Background Caspase-3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). The affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Synonyms: CPP32 ,CASP3, apopain, procaspase3,CPP32B,SCA-1,CPP-32,Apopain,Yama
Gene ID 836
UniProt P42574
Pathways Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation

Application Details

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Handling References for anti-Caspase 3 antibody (ABIN222873) Images back to top
Application Notes Western blotting (1:50 -1:500 dilutions) and Immunohistochemistry (1:10-1:20 dilutions). However, the optimal concentrations should be determined individually. The antibody preferentially recognizes the p17 fragment of the active caspase-3 in samples from human, mouse, and rat origins
Restrictions For Research Use only

Handling

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details References for anti-Caspase 3 antibody (ABIN222873) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg antigen affinity purified rabbit anti-Active Caspase-3 antibody in phosphate-buffered saline (PBS) containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-Caspase 3 antibody (ABIN222873)

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details Handling Images back to top
Product cited in:

Zahavi, Ionescu, Gluska, Gradus, Ben-Yaakov, Perlson: "A compartmentalized microfluidic neuromuscular co-culture system reveals spatial aspects of GDNF functions." in: Journal of cell science, Vol. 128, Issue 6, pp. 1241-52, 2015

Anjum, Krishna, Tsutsui: "Inhibitory roles of the mammalian GnIH ortholog RFRP3 in testicular activities in adult mice." in: The Journal of endocrinology, Vol. 223, Issue 1, pp. 79-91, 2014

Greenlee-Wacker, Rigby, Kobayashi, Porter, DeLeo, Nauseef: "Phagocytosis of Staphylococcus aureus by human neutrophils prevents macrophage efferocytosis and induces programmed necrosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 10, pp. 4709-17, 2014

Wang, Lauer, Anand, Mack, Maytin: "Hyaluronan synthase 2 protects skin fibroblasts against apoptosis induced by environmental stress." in: The Journal of biological chemistry, Vol. 289, Issue 46, pp. 32253-65, 2014

Sun, Li, He, Zhang, Tao: "Neuroprotective effect of minocycline in a rat model of branch retinal vein occlusion." in: Experimental eye research, Vol. 113, pp. 105-16, 2013

Eslami, Sharifi, Rahimi, Rahati: "Protective effect of telmisartan against oxidative damage induced by high glucose in neuronal PC12 cell." in: Neuroscience letters, 2013

Krejbich-Trotot, Denizot, Hoarau, Jaffar-Bandjee, Das, Gasque: "Chikungunya virus mobilizes the apoptotic machinery to invade host cell defenses." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 1, pp. 314-25, 2011

Finnie, Blumbergs, Manavis: "Aquaporin-4 expression after experimental contusional injury in an ovine impact-acceleration head injury model." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 18, Issue 7, pp. 947-50, 2011

Wu, Chen, Yen, Jayakumar, Chou, Hsiao, Sheu: "Neuroprotection by the Traditional Chinese Medicine, Tao-Hong-Si-Wu-Tang, against Middle Cerebral Artery Occlusion-Induced Cerebral Ischemia in Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2011, pp. 803015, 2010

Maher, Evans, Bottoms, Mercer, Thorley, Nicholson, Laurent, Tetley, Chambers, McAnulty: "Diminished prostaglandin E2 contributes to the apoptosis paradox in idiopathic pulmonary fibrosis." in: American journal of respiratory and critical care medicine, Vol. 182, Issue 1, pp. 73-82, 2010

Chang, Hsieh, Peng, Yen, Hsiao, Chou, Chen, Sheu: "Neuroprotective mechanisms of puerarin in middle cerebral artery occlusion-induced brain infarction in rats." in: Journal of biomedical science, Vol. 16, pp. 9, 2009

Frost, Zhou, Krasnesky, Armstrong: "Initiation of oligodendrocyte progenitor cell migration by a PDGF-A activated extracellular regulated kinase (ERK) signaling pathway." in: Neurochemical research, Vol. 34, Issue 1, pp. 169-81, 2009

Mendoza-Rodruiguez, Martuinez, Vargas, Nava, Morimoto, Espinosa, Cerbuon: "Mating modifies apoptosis pattern in epithelial cells of the rat uterus." in: Molecular reproduction and development, Vol. 76, Issue 6, pp. 564-72, 2009

Ou, Lee, Lee, Chiu, Tsai, Lin, Sheu: "Ginkgo biloba extract attenuates oxLDL-induced oxidative functional damages in endothelial cells." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 106, Issue 5, pp. 1674-85, 2009

Ueda, Ueyama, Oka, Ito, Tsuruo, Ichinose: "Polaprezinc (Zinc L-carnosine) is a potent inducer of anti-oxidative stress enzyme, heme oxygenase (HO)-1 - a new mechanism of gastric mucosal protection." in: Journal of pharmacological sciences, Vol. 110, Issue 3, pp. 285-94, 2009

Roy, Krishna: "Evidence of androgen-dependent sperm storage in female reproductive tract of Scotophilus heathi." in: General and comparative endocrinology, Vol. 165, Issue 1, pp. 120-6, 2009

Rabin, Flitton, Demuth: "Aggregatibacter actinomycetemcomitans cytolethal distending toxin induces apoptosis in nonproliferating macrophages by a phosphatase-independent mechanism." in: Infection and immunity, Vol. 77, Issue 8, pp. 3161-9, 2009

Guo, Tang, Li, Ji, Sun: "[The analysis of the treatment of giant cell tumor of the pelvis and sacrum]" in: Zhonghua wai ke za zhi [Chinese journal of surgery], Vol. 46, Issue 7, pp. 501-5, 2008

Schmidt, Paes, De Mazière, Smyczek, Yang, Gray, French, Kasman, Klumperman, Rice, Ye: "EGFL7 regulates the collective migration of endothelial cells by restricting their spatial distribution." in: Development (Cambridge, England), Vol. 134, Issue 16, pp. 2913-23, 2007

Engelhardt, Smith, Fodor: "Association of the influenza A virus RNA-dependent RNA polymerase with cellular RNA polymerase II." in: Journal of virology, Vol. 79, Issue 9, pp. 5812-8, 2005

Images

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details Handling References for anti-Caspase 3 antibody (ABIN222873) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (Cleavage Site), (N-Term), (active) antibody (ABIN222873) Western blot analysis of caspase-3 in camptothecin (2 uM) treated Jurkat cells
'Independent Validation' Badge
Antigen Caspase 3 (Cleavage Site)
Lot Number 1A273015
Method validated Immunohistochemistry
Positive Control Mouse cerebellar tissue samples at post natal day P5
Negative Control secondary antibody only
Notes The antibody works for IHC on frozen sections. Among the dilutions tested, 1:20 gave the best results. Both 1:10 and 1:50 dilutions gave a weaker signal respect to 1:20. The signal for Casp3 was detected both in cerebral cortex and cerebellum.
Secondary Antibody ABIN222873
Protocol
  • Mouse cerebellum at post natal day (P) 5 was perfusion fixed in paraformaldehyde 4% in 0.1M phosphate buffered saline (PBS) pH7.4 and post-fixed in the same fixative for 4 additional hours.
  • Following several washes in PBS, cerebellum was cryoprotected in 30% sucrose, frozen in liquid nitrogen and cut in 30μm thick sections.
  • Sections were rinsed in PBS for about 30min.
  • Sections were blocked in 0.01M PBS containing 0.1% Triton and 5% Normal Goat Serum (NGS) for 1h at RT.
  • Incubation with primary antibody ABIN222873 diluted 1:10/1:20/1:50 in the blocking solution ON at 4°C. The negative control slice was incubate with blocking solution only ON at 4°C.
  • Three 5min washed in 0.01M PBS at RT.
  • Incubation with the anti-rabbit secondary antibody (Invitrogen, AF488, Lot 1073084) diluted 1:800 in 0.1M PBS-0.1% Triton for 1h at RT.
  • Three 5min washed in 0.01M PBS at RT.
  • Specimens were mounted in Fluoroshield mounting medium (Sigma, F6182, Lot MKBX3783V).
  • Images were taken on a Leica SP5 upright confocal microscope (Magnification 40x, zoom 1.47).
Experimental Notes In order to exclude any nonspecific signal due to secondary antibody, for each experimental procedure, one control slice (negative control) was processed omitting the primary antibody: in this case the overnight incubation at 4°C degree was performed in diluent solution only.
Validation Images
Immunohistochemistry validation image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (Cleavage Site), (N-Term), (active) antibody (ABIN222873) P5 cerebellum, IHC on frozen slices 30 µm thick. Magnification 40x, zoom 1.47. A. Ant...