anti-Human Caspase 3 antibody for Western Blotting

Recommended Caspase 3 Antibody (supplied by: Log in to see )

Antigen
Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antibodies
  • CPP32
  • CPP32B
  • SCA-1
  • A830040C14Rik
  • AC-3
  • Apopain
  • CC3
  • Caspase-3
  • Lice
  • Yama
  • mldy
  • xcpp32
  • casp3
  • zgc:100890
  • CASP-3
  • caspase-3
  • caspase 3
  • caspase 3 S homeolog
  • caspase 3, apoptosis-related cysteine peptidase a
  • caspase 3, apoptosis-related cysteine peptidase
  • CASP3
  • Casp3
  • casp3.S
  • casp3a
Reactivity
Human, Mouse (Murine), Rat (Rattus)
587
326
272
45
44
34
29
25
22
19
17
16
15
11
8
7
6
4
2
2
Host
Mouse
501
145
19
4
2
Clonality
Monoclonal
Conjugate
This Caspase 3 antibody is un-conjugated
31
26
19
11
10
9
8
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
516
278
208
157
77
72
63
58
48
29
28
23
13
9
6
5
3
3
2
2
2
2
2
2
1
1
1
1
Options
Supplier
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Available images

Catalog No. ABIN3188045
$ 181.73
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
101.5367 ABIN222873 IHC WB Rabbit IgG Cleavage Site, N-Term, active Log in to see Polyclonal 24
55.808132 ABIN3043802 IHC (p) WB Rabbit IgG AA 67-175 Log in to see Polyclonal 103
46.808132 ABIN724625 IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 60-100 Log in to see Polyclonal 65
43.808132 ABIN3044357 ICC IHC (p) WB Rabbit IgG AA 220-236, C-Term Log in to see Polyclonal 94
43.34621 ABIN252651 CyTOF EM FACS ICC IF IHC IHC (fro) IHC (p) SimWes WB Mouse IgG1 kappa Log in to see 31A1067 114
37.808132 ABIN3042980 WB Rabbit IgG AA 70-90, N-Term Log in to see Polyclonal 93
36.39226 ABIN3023067 IP WB Rabbit IgG Log in to see Polyclonal 1
31.808132 ABIN3043752 WB Rabbit IgG AA 207-220, C-Term Log in to see Polyclonal 93
28.808132 ABIN6260481 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 7
27.308132 ABIN6269461 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 12
24.308132 ABIN6254994 ELISA ICC IF IHC WB Rabbit IgG pSer150 Log in to see Polyclonal 1
23.5 ABIN747728 IF (p) IHC (p) WB Rabbit IgG AA 1-45 Log in to see Polyclonal 3
22.892262 ABIN3183667 ELISA IF IHC WB Rabbit IgG Ser1981 Log in to see Polyclonal 0
22.892262 ABIN5542609 ELISA FACS ICC IHC WB Mouse IgG1 AA 29-175 Log in to see 3D4D10 0
22.892262 ABIN5542610 ELISA FACS ICC IHC WB Mouse IgG1 AA 29-175 Log in to see 3D4D7 0
19.892262 ABIN3181575 IHC WB Rabbit IgG Log in to see Polyclonal 0
19.892262 ABIN3020698 IHC WB Rabbit IgG Log in to see Polyclonal 0
19.808132 ABIN1871461 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
19.808132 ABIN1980380 ICC IHC WB Rabbit IgG AA 29-175 Log in to see Polyclonal 0
19.808132 ABIN2899469 IF/ICC IHC IP WB Rabbit AA 29-175 Log in to see Polyclonal 0

General

Antigen Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(587), (326), (272), (45), (44), (34), (29), (25), (22), (19), (17), (16), (15), (11), (8), (7), (6), (4), (2), (2)
Host Mouse
(501), (145), (19), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 3 antibody is un-conjugated
(31), (26), (19), (11), (10), (9), (8), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(516), (278), (208), (157), (77), (72), (63), (58), (48), (29), (28), (23), (13), (9), (6), (5), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-Caspase 3 Antibody

Target Details Caspase 3 Application Details Handling Images
Specificity The antibody detects endogenous Active Caspase-3 protein.
Characteristics Mouse Monoclonal to Active Caspase-3.
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Immunogen Recombinant Protein
Clone 5E1
Isotype IgG1
Plasmids, Primers & others

Target Details Caspase 3

Product Details anti-Caspase 3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Caspase-3 (CASP3 Antibody Abstract)
Gene ID 836
UniProt P42574
Pathways Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation

Application Details

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Handling Images back to top
Application Notes WB 1:500-1000, IHC 1:100-200
Restrictions For Research Use only

Handling

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details Images back to top
Format Liquid
Buffer Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze/thaw cycles.
Storage -20 °C
Storage Comment Store at -20°C.

Images

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN3188045) anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody
Immunohistochemistry (IHC) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN3188045) Immunohistochemistry (IHC) analysis of paraffin-embedded Human Tonsil Tissue using Ac...
'Independent Validation' Badge
Antigen Caspase 3 (active)
Method validated Western Blotting
Positive Control Mouse cerebellar tissue samples at post natal day P5
Notes The antibody recognized both the active and non-active form of Caspase3 and worked well in the experimental conditions tested. The product is therefore indicated for the WB use.
Secondary Antibody ABIN3179097
Protocol
  • Mouse cerebellar tissue samples at post natal day (P) 5 were lysed in 150 µL of lysis buffer.
  • Protein concentration was determined using Bradford reagen (Sigma B6916) according to the manufacturer's recommendation.
  • 30 and 60µg total protein were denatured in sample buffer for 5min at 95°C and then cooled on ice.
  • Proteins were separated on a denaturing homemade 15% SDS-PAGE gel at a constant voltage of 180V for 70min.
  • Proteins were transferred in a transfer buffer to nitrocellulose membrane (Amersham Biosciences, RPN203D, lot 18100/66/0207) at constant amperage of 400mA for 60min.
  • Blocking of the membrane with blocking solution (0.01M PBS, 0.1% tween, 2% Bovine Serum Albumin) for 1h at RT.
  • Incubation with the primary antibody ABIN3179097 diluted 1:1000 in blocking solution ON at 4°C.
  • 3 washes in PBS-0.1% tween for 15min at RT.
  • Incubation with the anti-mouse horseradish peroxidase (HRP) secondary antibody (Santa Cruz, SC2005, lot H0406) diluted 1:10000 in blocking solution for 1h at RT.
  • Washes in PBS-0.1% tween for 15min at RT.
  • Chemiluminescence detection using homemade ECL (1min incubation at RT) and revealed on HYPERFILM EGL (Amersham Biosciences, RPN2013K, lot 04450300115); exposure 30sec.
  • Proteins were revealed using the ECL technique on western blot films for 30 seconds.
Experimental Notes
  • A band around 30-35 kDa and a band around 17kDa were detected with the experimental procedures previously described.
  • A protein load of 30µg is recommended, as in the 60µg load, weak non-specific bands were detected around 15 and 30kDa.
Validation Images
Western Blotting validation image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN3179097) P5 mouse cerebellum, 30 seconds exposition. 60µg (left) and 30µg (right) protein load...