anti-Rat (Rattus) Caspase 3 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Caspase 3 Antibody (supplied by: Log in to see )

Antigen
Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antibodies
  • CPP32
  • CPP32B
  • SCA-1
  • A830040C14Rik
  • AC-3
  • Apopain
  • CC3
  • Caspase-3
  • Lice
  • Yama
  • mldy
  • xcpp32
  • CASP-3
  • caspase-3
  • caspase 3
  • caspase 3 S homeolog
  • caspase 3, apoptosis-related cysteine peptidase
  • CASP3
  • Casp3
  • casp3.S
Reactivity
Chinese Hamster, Human, Mammalian, Mouse (Murine), Pig (Porcine), Rat (Rattus)
634
352
299
46
44
34
30
25
22
19
18
16
15
11
7
7
6
4
2
2
Host
Mouse
509
184
19
4
2
Clonality
Monoclonal
Conjugate
This Caspase 3 antibody is un-conjugated
33
28
21
15
13
10
9
6
6
5
5
5
5
5
5
5
5
4
3
3
3
2
2
2
2
2
2
2
2
2
1
Application
Cytometry by Time of Flight (CyTOF), Electron Microscopy (EM), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
556
310
245
162
104
77
65
58
50
29
28
23
13
9
6
4
3
3
2
2
2
2
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN252651
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10.879798 ABIN5663167 IHC IHC (p) SimWes WB Mouse IgG2a kappa Log in to see CPP32 4-1-18 18
10.879798 ABIN724625 IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 60-100 Log in to see Polyclonal 65
10.879798 ABIN4288091 ICC IF IHC (p) IHC Rabbit IgG Log in to see Polyclonal 11
10.879798 ABIN747728 IF (p) IHC (p) WB Rabbit IgG AA 1-45 Log in to see Polyclonal 3
10.879798 ABIN4288095 FACS IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 16
10.879798 ABIN4288099 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
10.879798 ABIN615023 IHC (p) IP WB Rabbit IgG Log in to see Polyclonal 0
10.879798 ABIN4288100 ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit IgG Center Log in to see Polyclonal 0
10.879798 ABIN364239 IHC IHC (p) IP WB Rabbit IgG Log in to see Polyclonal 0
10.879798 ABIN462092 IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
10.879798 ABIN498946 EIA IHC (p) WB Rabbit Log in to see Polyclonal 0
10.879798 ABIN615153 IHC (p) WB Rabbit Log in to see Polyclonal 0
10.879798 ABIN4288120 ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see 31A1067 0
1 ABIN3043802 IHC (p) WB Rabbit IgG AA 67-175 Log in to see Polyclonal 68
1 ABIN5518695 IHC (p) WB Rabbit IgG AA 67-81, N-Term Log in to see Polyclonal 67
1 ABIN5518656 IHC (p) WB Rabbit IgG AA 220-236, C-Term Log in to see Polyclonal 67
1 ABIN126957 IHC (p) WB Rabbit Cleavage Site, N-Term, active Log in to see Polyclonal 0
1 ABIN5563858 IHC (p) IHC WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN4901138 IHC (p) Mouse IgG1 AA 20-100 Log in to see 4B8 0
1 ABIN4901139 IHC (p) Mouse IgG1 AA 20-100 Log in to see 5H10 0

General

Antigen Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antibodies
Reactivity Chinese Hamster, Human, Mammalian, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(634), (352), (299), (46), (44), (34), (30), (25), (22), (19), (18), (16), (15), (11), (7), (7), (6), (4), (2), (2)
Host Mouse
(509), (184), (19), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 3 antibody is un-conjugated
(33), (28), (21), (15), (13), (10), (9), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Cytometry by Time of Flight (CyTOF), Electron Microscopy (EM), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(556), (310), (245), (162), (104), (77), (65), (58), (50), (29), (28), (23), (13), (9), (6), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 110 references available
Supplier Log in to see

Product Details anti-Caspase 3 Antibody

Target Details Caspase 3 Application Details Handling References for anti-Caspase 3 antibody (ABIN252651) Images
Specificity The antibody detects both pro Caspase-3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this antibody in Western blot applications.
Purification Protein G purified
Immunogen Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.
Clone 31A1067
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details Caspase 3

Product Details anti-Caspase 3 Antibody Application Details Handling References for anti-Caspase 3 antibody (ABIN252651) Images back to top
Antigen
Alternative Name Caspase-3 (CASP3 Antibody Abstract)
Background Gene Symbol: CASP3
Gene ID 836
Pathways Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation

Application Details

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Handling References for anti-Caspase 3 antibody (ABIN252651) Images back to top
Application Notes Western Blot 1 - 5 μg/mL, Simple Western 1:50, Flow Cytometry, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:10 - 1:500, Electron Microscopy, CyTOF-readyUseful in Immunohistochemistry-Frozen See Zhang et al., and Immunohistochemistry-Paraffin See Lee et al. Use in Immunocytochemistry/Immunofluorescence was reported in the scientific literature (PMID: 23840553). Use in FLOW cytometry reported in scientific literature ( PMID 27429862). Use in electron microscopy reported in scientific literature ( PMID 27450722). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details References for anti-Caspase 3 antibody (ABIN252651) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Caspase 3 antibody (ABIN252651)

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details Handling Images back to top
Product cited in:

Sakchaisri, Kim, Hwang, Soung, Lee, Choi, Lee, Park, Thimmegowda, Lee, Shwetha, Srinivasrao, Pham, Jang, Yum, Surh, Lee, Park, Kim, Kwon, Ahn, Kim: "Anticancer activity of a novel small molecule tubulin inhibitor STK899704." in: PLoS ONE, Vol. 12, Issue 3, pp. e0173311, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Chao, Chen, Huang, Chang, HuangFu, Lee, Teng, Pan: "Lanatoside C, a cardiac glycoside, acts through protein kinase Cδ to cause apoptosis of human hepatocellular carcinoma cells." in: Scientific reports, Vol. 7, pp. 46134, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Chang, Liu, Chiu, Chen, Chuang, Chen, Hsu, Lu, Chiou, Chou, Chiu: "A 4-Phenoxyphenol Derivative Exerts Inhibitory Effects on Human Hepatocellular Carcinoma Cells through Regulating Autophagy and Apoptosis Accompanied by Downregulating α-Tubulin Expression." in: Molecules (Basel, Switzerland), Vol. 22, Issue 5, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kao, Chen, Guo, Feng, Hung, Yang, Sung, Tsui, Chu, Chen, Wen: "The 1-Tosylpentan-3-one Protects against 6-Hydroxydopamine-Induced Neurotoxicity." in: International journal of molecular sciences, Vol. 18, Issue 5, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Schecher, Walter, Falkenstein, Macher-Goeppinger, Stenzel, Krümpelmann, Hadaschik, Perner, Kristiansen, Duensing, Roth, Tagscherer: "Cyclin K dependent regulation of Aurora B affects apoptosis and proliferation by induction of mitotic catastrophe in prostate cancer." in: International journal of cancer, Vol. 141, Issue 8, pp. 1643-1653, 2017 (Sample species: Human).

Palavicini, Lloyd, Hayes, Bianchi, Kang, Dawson-Scully, Lakshmana: "RanBP9 Plays a Critical Role in Neonatal Brain Development in Mice." in: PLoS ONE, Vol. 8, Issue 6, pp. e66908, 2016 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Ge, Xia, He, Zhang, Liu, Shao, Wang, Xiao, Ge, Li, Li, Chen: "Generation and characterization of a breast carcinoma model by PyMT overexpression in mammary epithelial cells of tree shrew, an animal close to primates in evolution." in: International journal of cancer, Vol. 138, Issue 3, pp. 642-51, 2016 (Sample species: Mammalian). Further details: Western Blotting

Han, Chiang, Lin, Tang, Lee, Huang, Zeng, Chuang, Huang: "Comparison of Immunomodulatory and Anticancer Activities in Different Strains of Tremella fuciformis Berk." in: The American journal of Chinese medicine, Vol. 43, Issue 8, pp. 1637-55, 2016 (Sample species: Human). Further details: Western Blotting

Chen, Chyau, Lee, Tseng, Chou: "Promotion of mitotic catastrophe via activation of PTEN by paclitaxel with supplement of mulberry water extract in bladder cancer cells." in: Scientific reports, Vol. 6, pp. 20417, 2016 (Sample species: Human). Further details: Western Blotting

Repsold, Pretorius, Joubert: "Ex vivo apoptotic and autophagic influence of an estradiol analogue on platelets." in: Experimental hematology & oncology, Vol. 5, pp. 18, 2016 (Sample species: Human). Further details: Flow Cytometry

Shi, Oh, Zhang, Yao, Yue, Li, Kanteti, Riehm, Salgia, Owonikoko, Ramalingam, Chen, Sun: "Met gene amplification and protein hyperactivation is a mechanism of resistance to both first and third generation EGFR inhibitors in lung cancer treatment." in: Cancer letters, Vol. 380, Issue 2, pp. 494-504, 2016 (Sample species: Human). Further details: Immunoelectron Microscopy,Flow Cytometry

Huang, Chao, Li, Chang, Chen, Chen, Cheng, Liou, Teng, Pan: "MPT0G066, a novel anti-mitotic drug, induces JNK-independent mitotic arrest, JNK-mediated apoptosis, and potentiates antineoplastic effect of cisplatin in ovarian cancer." in: Scientific reports, Vol. 6, pp. 31664, 2016 (Sample species: Human). Further details: Western Blotting

Huang, Lien, Chen, Liu, Wang, Ping, Lin, Huang, Lin: "Antiviral Activity of a Novel Compound CW-33 against Japanese Encephalitis Virus through Inhibiting Intracellular Calcium Overload." in: International journal of molecular sciences, Vol. 17, Issue 9, 2016 (Sample species: Human). Further details: Western Blotting

Liu, Lv, Zou, Liu, Ma, Wang, Sa, Jing, Xu: "Afatinib down-regulates MCL-1 expression through the PERK-eIF2α-ATF4 axis and leads to apoptosis in head and neck squamous cell carcinoma." in: American journal of cancer research, Vol. 6, Issue 8, pp. 1708-19, 2016 (Sample species: Human). Further details: Western Blotting

Oliver Metzig, Fuchs, Tagscherer, Gröne, Schirmacher, Roth: "Inhibition of caspases primes colon cancer cells for 5-fluorouracil-induced TNF-α-dependent necroptosis driven by RIP1 kinase and NF-κB." in: Oncogene, Vol. 35, Issue 26, pp. 3399-409, 2016 (Sample species: Human). Further details: Western Blotting

Huang, Huang, Gou, Wang, Liao, Wang: "Combretastatin A-4 Analogue: A Dual-Targeting and Tubulin Inhibitor Containing Antitumor Pt(IV) Moiety with a Unique Mode of Action." in: Bioconjugate chemistry, Vol. 27, Issue 9, pp. 2132-48, 2016 (Sample species: Human). Further details: Western Blotting

Patel, Garikapati, Ramaiah, Polavarapu, Bhadra, Bhadra: "miR-15a/miR-16 induces mitochondrial dependent apoptosis in breast cancer cells by suppressing oncogene BMI1." in: Life sciences, Vol. 164, pp. 60-70, 2016 (Sample species: Human). Further details: Western Blotting

Nepali, Lee, Lai, Ojha, Wu, Wu, Chen, Liou: "Ring-opened tetrahydro-γ-carbolines display cytotoxicity and selectivity with histone deacetylase isoforms." in: European journal of medicinal chemistry, Vol. 127, pp. 115-127, 2016 Method employed by authors: Flow Cytometry (FACS), Western Blotting (WB) (Sample species: Human).

Ren, Guo, Thakur, Zhang, Wang, Liang, Shi, Gao, Liu, Feng, Chen, Yang, Shang, Liu, Xu, Chen: "Blockade efficacy of MEK/ERK-dependent autophagy enhances PI3K/Akt inhibitor NVP-BKM120's therapeutic effectiveness in lung cancer cells." in: Oncotarget, Vol. 7, Issue 41, pp. 67277-67287, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kwon, Willy, Quirin, Wek, Korc, Yin, Kota: "Novel role of miR-29a in pancreatic cancer autophagy and its therapeutic potential." in: Oncotarget, Vol. 7, Issue 44, pp. 71635-71650, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details Handling References for anti-Caspase 3 antibody (ABIN252651) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Western blot analysis of Caspase-3 in HeLa cells. Cells were treated with 2 mM stauro...
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Western blot analysis of Caspase-3 in multiple human tissues using ABIN252651 at 2 ug...
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Western blot analysis of Caspase-3 in mouse RAW cells.
Simple Western (SimWes) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - Simpl...
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - Analysi...
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - Analysi...
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - Analysi...
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - Lanes 1...
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - analysi...
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - Western...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2...
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) - Lysates of Jurkat hu...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) - Cas...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) - IHC...
Flow Cytometry (FACS) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody (ABIN252651) Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) - An intracellular s...