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|Antigen||Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antibodies|
|Reactivity||Chinese Hamster, Human, Mammalian, Mouse (Murine), Pig (Porcine), Rat (Rattus) Alternatives|
|Conjugate||This Caspase 3 antibody is un-conjugated Alternatives|
Cytometry by Time of Flight (CyTOF), Electron Microscopy (EM), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
|114 references available|
|Supplier||Log in to see|
Product Details anti-Caspase 3 AntibodyTarget Details Caspase 3 Application Details Handling References for anti-Caspase 3 antibody (ABIN252651) Images
|Specificity||The antibody detects both pro Caspase-3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this antibody in Western blot applications.|
|Purification||Protein G purified|
|Immunogen||Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.|
|Plasmids, Primers & others|
Target Details Caspase 3Product Details anti-Caspase 3 Antibody Application Details Handling References for anti-Caspase 3 antibody (ABIN252651) Images back to top
|Alternative Name||Caspase-3 (CASP3 Antibody Abstract)|
|Background||Gene Symbol: CASP3|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation|
Application DetailsProduct Details anti-Caspase 3 Antibody Target Details Caspase 3 Handling References for anti-Caspase 3 antibody (ABIN252651) Images back to top
|Application Notes||Western Blot 1 - 5 μg/mL, Simple Western 1:50, Flow Cytometry, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:10 - 1:500, Electron Microscopy, CyTOF-readyUseful in Immunohistochemistry-Frozen See Zhang et al., and Immunohistochemistry-Paraffin See Lee et al. Use in Immunocytochemistry/Immunofluorescence was reported in the scientific literature (PMID: 23840553). Use in FLOW cytometry reported in scientific literature ( PMID 27429862). Use in electron microscopy reported in scientific literature ( PMID 27450722). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details References for anti-Caspase 3 antibody (ABIN252651) Images back to top
Buffer contains: 0.05 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-Caspase 3 antibody (ABIN252651)Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details Handling Images back to top
|Product cited in:||
Krishnamoorthy, Khanna, Parnaik: "E3 ubiquitin ligase HECW2 targets PCNA and lamin B1." in: Biochimica et biophysica acta. Molecular cell research, Vol. 1865, Issue 8, pp. 1088-1104, 2018
Chou, Liu, Hong, Dahms, Chiu, Chang, Chien, Yen, Cheng, Chiu: "Ethyl Acetate Extract of Scindapsus cf. hederaceus Exerts the Inhibitory Bioactivity on Human Non-Small Cell Lung Cancer Cells through Modulating ER Stress." in: International journal of molecular sciences, Vol. 19, Issue 7, 2018
Lin, Wang, Lin, Leu, Cheng, Chen, Hwang: "Synergistic effects of tetrandrine combined with ionizing radiation on a murine colorectal carcinoma‑bearing mouse model." in: Oncology reports, Vol. 40, Issue 3, pp. 1390-1400, 2018
Sakchaisri, Kim, Hwang, Soung, Lee, Choi, Lee, Park, Thimmegowda, Lee, Shwetha, Srinivasrao, Pham, Jang, Yum, Surh, Lee, Park, Kim, Kwon, Ahn, Kim: "Anticancer activity of a novel small molecule tubulin inhibitor STK899704." in: PLoS ONE, Vol. 12, Issue 3, pp. e0173311, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).
Chao, Chen, Huang, Chang, HuangFu, Lee, Teng, Pan: "Lanatoside C, a cardiac glycoside, acts through protein kinase Cδ to cause apoptosis of human hepatocellular carcinoma cells." in: Scientific reports, Vol. 7, pp. 46134, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).
Chang, Liu, Chiu, Chen, Chuang, Chen, Hsu, Lu, Chiou, Chou, Chiu: "A 4-Phenoxyphenol Derivative Exerts Inhibitory Effects on Human Hepatocellular Carcinoma Cells through Regulating Autophagy and Apoptosis Accompanied by Downregulating α-Tubulin Expression." in: Molecules (Basel, Switzerland), Vol. 22, Issue 5, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).
Kao, Chen, Guo, Feng, Hung, Yang, Sung, Tsui, Chu, Chen, Wen: "The 1-Tosylpentan-3-one Protects against 6-Hydroxydopamine-Induced Neurotoxicity." in: International journal of molecular sciences, Vol. 18, Issue 5, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).
Schecher, Walter, Falkenstein, Macher-Goeppinger, Stenzel, Krümpelmann, Hadaschik, Perner, Kristiansen, Duensing, Roth, Tagscherer: "Cyclin K dependent regulation of Aurora B affects apoptosis and proliferation by induction of mitotic catastrophe in prostate cancer." in: International journal of cancer, Vol. 141, Issue 8, pp. 1643-1653, 2017 (Sample species: Human).
Palavicini, Lloyd, Hayes, Bianchi, Kang, Dawson-Scully, Lakshmana: "RanBP9 Plays a Critical Role in Neonatal Brain Development in Mice." in: PLoS ONE, Vol. 8, Issue 6, pp. e66908, 2016 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry
Ge, Xia, He, Zhang, Liu, Shao, Wang, Xiao, Ge, Li, Li, Chen: "Generation and characterization of a breast carcinoma model by PyMT overexpression in mammary epithelial cells of tree shrew, an animal close to primates in evolution." in: International journal of cancer, Vol. 138, Issue 3, pp. 642-51, 2016 (Sample species: Mammalian). Further details: Western Blotting
Han, Chiang, Lin, Tang, Lee, Huang, Zeng, Chuang, Huang: "Comparison of Immunomodulatory and Anticancer Activities in Different Strains of Tremella fuciformis Berk." in: The American journal of Chinese medicine, Vol. 43, Issue 8, pp. 1637-55, 2016 (Sample species: Human). Further details: Western Blotting
Chen, Chyau, Lee, Tseng, Chou: "Promotion of mitotic catastrophe via activation of PTEN by paclitaxel with supplement of mulberry water extract in bladder cancer cells." in: Scientific reports, Vol. 6, pp. 20417, 2016 (Sample species: Human). Further details: Western Blotting
Repsold, Pretorius, Joubert: "Ex vivo apoptotic and autophagic influence of an estradiol analogue on platelets." in: Experimental hematology & oncology, Vol. 5, pp. 18, 2016 (Sample species: Human). Further details: Flow Cytometry
Shi, Oh, Zhang, Yao, Yue, Li, Kanteti, Riehm, Salgia, Owonikoko, Ramalingam, Chen, Sun: "Met gene amplification and protein hyperactivation is a mechanism of resistance to both first and third generation EGFR inhibitors in lung cancer treatment." in: Cancer letters, Vol. 380, Issue 2, pp. 494-504, 2016 (Sample species: Human). Further details: Immunoelectron Microscopy,Flow Cytometry
Huang, Chao, Li, Chang, Chen, Chen, Cheng, Liou, Teng, Pan: "MPT0G066, a novel anti-mitotic drug, induces JNK-independent mitotic arrest, JNK-mediated apoptosis, and potentiates antineoplastic effect of cisplatin in ovarian cancer." in: Scientific reports, Vol. 6, pp. 31664, 2016 (Sample species: Human). Further details: Western Blotting
Huang, Lien, Chen, Liu, Wang, Ping, Lin, Huang, Lin: "Antiviral Activity of a Novel Compound CW-33 against Japanese Encephalitis Virus through Inhibiting Intracellular Calcium Overload." in: International journal of molecular sciences, Vol. 17, Issue 9, 2016 (Sample species: Human). Further details: Western Blotting
Liu, Lv, Zou, Liu, Ma, Wang, Sa, Jing, Xu: "Afatinib down-regulates MCL-1 expression through the PERK-eIF2α-ATF4 axis and leads to apoptosis in head and neck squamous cell carcinoma." in: American journal of cancer research, Vol. 6, Issue 8, pp. 1708-19, 2016 (Sample species: Human). Further details: Western Blotting
Oliver Metzig, Fuchs, Tagscherer, Gröne, Schirmacher, Roth: "Inhibition of caspases primes colon cancer cells for 5-fluorouracil-induced TNF-α-dependent necroptosis driven by RIP1 kinase and NF-κB." in: Oncogene, Vol. 35, Issue 26, pp. 3399-409, 2016 (Sample species: Human). Further details: Western Blotting
Huang, Huang, Gou, Wang, Liao, Wang: "Combretastatin A-4 Analogue: A Dual-Targeting and Tubulin Inhibitor Containing Antitumor Pt(IV) Moiety with a Unique Mode of Action." in: Bioconjugate chemistry, Vol. 27, Issue 9, pp. 2132-48, 2016 (Sample species: Human). Further details: Western Blotting
Patel, Garikapati, Ramaiah, Polavarapu, Bhadra, Bhadra: "miR-15a/miR-16 induces mitochondrial dependent apoptosis in breast cancer cells by suppressing oncogene BMI1." in: Life sciences, Vol. 164, pp. 60-70, 2016 (Sample species: Human). Further details: Western Blotting
ImagesProduct Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details Handling References for anti-Caspase 3 antibody (ABIN252651) back to top