anti-Rat (Rattus) Caspase 3 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Caspase 3 Antibody (supplied by: Log in to see )

Antigen
Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antibodies
  • CPP32
  • CPP32B
  • SCA-1
  • A830040C14Rik
  • AC-3
  • Apopain
  • CC3
  • Caspase-3
  • Lice
  • Yama
  • mldy
  • xcpp32
  • casp3
  • zgc:100890
  • CASP-3
  • caspase-3
  • caspase 3
  • caspase 3 S homeolog
  • caspase 3, apoptosis-related cysteine peptidase a
  • caspase 3, apoptosis-related cysteine peptidase
  • CASP3
  • Casp3
  • casp3.S
  • casp3a
Epitope
AA 67-175
41
31
30
28
25
20
18
16
15
15
13
12
11
11
10
10
8
7
6
6
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
596
328
274
45
44
34
29
25
22
19
17
16
15
11
8
7
6
4
2
2
Host
Rabbit
508
147
19
4
2
Clonality
Polyclonal
Conjugate
This Caspase 3 antibody is un-conjugated
31
26
19
11
10
9
8
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
523
286
209
155
80
73
67
58
48
29
28
23
13
9
6
5
3
3
2
2
2
2
2
2
1
1
1
1
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Catalog No. ABIN3043802
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
50.755672 ABIN724625 IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 60-100 Log in to see Polyclonal 65
45.99742 ABIN252651 CyTOF EM FACS ICC IF IHC IHC (fro) IHC (p) SimWes WB Mouse IgG1 kappa Log in to see 31A1067 114
37 ABIN5518695 IHC (p) WB Rabbit IgG AA 67-81, N-Term Log in to see Polyclonal 94
28.255672 ABIN747728 IF (p) IHC (p) WB Rabbit IgG AA 1-45 Log in to see Polyclonal 3
20.497421 ABIN5663167 IHC IHC (p) SimWes WB Mouse IgG2a kappa Log in to see CPP32 4-1-18 18
14.7556715 ABIN364239 IHC IHC (p) IP WB Rabbit IgG Log in to see Polyclonal 0
14.5 ABIN537395 IHC (p) IP WB Rabbit Log in to see Polyclonal 1
14.497421 ABIN4288099 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
13 ABIN3030302 FACS IHC (p) WB Rabbit IgG AA 67-175 Log in to see Polyclonal 0
12.811332 ABIN4901138 IHC (p) Mouse IgG1 AA 20-100 Log in to see 4B8 0
12.811332 ABIN4901139 IHC (p) Mouse IgG1 AA 20-100 Log in to see 5H10 0
11.7556715 ABIN615023 IHC (p) IP WB Rabbit IgG Log in to see Polyclonal 0
11.7556715 ABIN462092 IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
11.7556715 ABIN498946 EIA IHC (p) WB Rabbit Log in to see Polyclonal 0
11.497421 ABIN4288095 FACS IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 16
9.997421 ABIN4288120 ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see 31A1067 0
8.7556715 ABIN615153 IHC (p) WB Rabbit Log in to see Polyclonal 0
8.497421 ABIN4288091 ICC IF IHC (p) IHC Rabbit IgG Log in to see Polyclonal 13
7 ABIN2891155 ELISA IHC IHC (p) WB Rabbit IgG AA 126-175, Cleaved-Asp175 Log in to see Polyclonal 0
7 ABIN957188 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 0

General

Antigen Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antibodies
Epitope AA 67-175
(41), (31), (30), (28), (25), (20), (18), (16), (15), (15), (13), (12), (11), (11), (10), (10), (8), (7), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(596), (328), (274), (45), (44), (34), (29), (25), (22), (19), (17), (16), (15), (11), (8), (7), (6), (4), (2), (2)
Host Rabbit
(508), (147), (19), (4), (2)
Clonality
Polyclonal
Conjugate This Caspase 3 antibody is un-conjugated
(31), (26), (19), (11), (10), (9), (8), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(523), (286), (209), (155), (80), (73), (67), (58), (48), (29), (28), (23), (13), (9), (6), (5), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 103 references available
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Product Details anti-Caspase 3 Antibody

Target Details Caspase 3 Application Details Handling References for anti-Caspase 3 antibody (ABIN3043802) Images
Purpose Rabbit IgG polyclonal antibody for Caspase-3(CASP3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Caspase-3(CASP3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: caspase 3, apoptosis-related cysteine peptidase
Protein Name: Caspase-3
Purification Immunogen affinity purified.
Immunogen E.coli-derived human Caspase 3 recombinant protein (Position: T67-D175). Human Caspase 3 shares 86% and 90% amino acid (aa) sequences identity with mouse and rat Caspase 3, respectively.
Isotype IgG
Plasmids, Primers & others

Target Details Caspase 3

Product Details anti-Caspase 3 Antibody Application Details Handling References for anti-Caspase 3 antibody (ABIN3043802) Images back to top
Antigen
Alternative Name CASP3 (CASP3 Antibody Abstract)
Background Caspase 3 is a caspase protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located on 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.

Synonyms: A830040C14Rik antibody|Apopain antibody|Apopain precursor antibody|CASP 3 antibody|CASP-3 antibody|CASP3 antibody|CASP3_HUMAN antibody|Casp3a antibody|Caspase 3 antibody|Caspase 3 apoptosis related cysteine protease antibody|Caspase 3 p12 subunit antibody|Caspase 3 p17 subunit antibody|Caspase 3, apoptosis-related cysteine peptidase antibody|Caspase 3, apoptosis-related cysteine protease antibody|Caspase 3, apoptosis-related cysteine protease a antibody|Caspase-3 subunit p12 antibody|Caspase3 antibody|CC3 antibody|CPP 32 antibody|CPP-32 antibody|CPP32 antibody|CPP32B antibody|Cysteine protease CPP32 antibody| EC 3.4.22.56 antibody|Human cysteine protease CPP32 isoform alpha mRNA complete cds antibody|ICE3 antibody|LICE antibody|mldy antibody|OTTHUMP00000165052 antibody|OTTHUMP00000165053 antibody|OTTHUMP00000165054 antibody|PARP cleavage protease antibody|Procaspase3 antibody|Protein Yama antibody|SCA 1 antibody|SCA-1 antibody|SCA1 antibody|SREBP cleavage activity 1 antibody|Yama antibody|Yama protein antibody
Gene ID 836
UniProt P42574
Pathways Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation

Application Details

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Handling References for anti-Caspase 3 antibody (ABIN3043802) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for Caspase 3 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details References for anti-Caspase 3 antibody (ABIN3043802) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Caspase 3 antibody (ABIN3043802)

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details Handling Images back to top
Product cited in:

An, Liu, She, Wu, Tian, Shi, Hao, Ren, Yang, Lu, Yang, Wu: "Replication of hepatitis E virus in the ovary and promotion of oocyte apoptosis in rabbits infected with HEV-4." in: Oncotarget, Vol. 9, Issue 4, pp. 4475-4484, 2018

Shi, Wang, Zhao, Long, Deng, Wang: "Bone marrow mesenchymal stem cell-derived exosomal miR-21 protects C-kit+ cardiac stem cells from oxidative injury through the PTEN/PI3K/Akt axis." in: PLoS ONE, Vol. 13, Issue 2, pp. e0191616, 2018

Bai, Yang, Luo: "Effects of 5-hydroxy-4'-nitro-7-propionyloxy-genistein on inhibiting proliferation and invasion via activating reactive oxygen species in human ovarian cancer A2780/DDP cells." in: Oncology letters, Vol. 15, Issue 4, pp. 5227-5235, 2018

Yang, Shi, Soomro, Hu, Du, She: "Hepatitis E Virus Induces Hepatocyte Apoptosis via Mitochondrial Pathway in Mongolian Gerbils." in: Frontiers in microbiology, Vol. 9, pp. 460, 2018

Wang, Li, Yang, Gao, Lin, Wang, Zhou, Hu: "Ginsenoside Rb1 inhibit apoptosis in rat model of Alzheimer's disease induced by Aβ1-40." in: American journal of translational research, Vol. 10, Issue 3, pp. 796-805, 2018

Qi, Xue, Lv, Sun, Du, Cai, Li, Gu, Wang: "Ginkgolic acids induce HepG2 cell death via a combination of apoptosis, autophagy and the mitochondrial pathway." in: Oncology letters, Vol. 15, Issue 5, pp. 6400-6408, 2018

Gou, Tian, Kong, Chen, Li, Lv, Zhang: "Salmon Calcitonin Attenuates Degenerative Changes in Cartilage and Subchondral Bone in Lumbar Facet Joint in an Experimental Rat Model." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 2849-2857, 2018

Shi, Ma, Ren, Liang, Yu, Luo: "Antitumorpharmacological mechanism of the oral liquid of Poriacocos polysaccharide." in: Journal of ethnopharmacology, Vol. 209, pp. 24-31, 2017

Xu, Xie, Wang, Wang, Luo, Zhou, Wang, Huang, Tan, Jin, Qi, Tang, Chen, Du, Zhao, Liang, Chen: "A novel fibroblast growth factor receptor 1 inhibitor protects against cartilage degradation in a murine model of osteoarthritis." in: Scientific reports, Vol. 6, pp. 24042, 2017

Hu, Sun, Zhang, Zhang, Hu: "Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway." in: Bioscience reports, Vol. 36, Issue 3, 2017

Zhou, He, Wang, Xie, Liu, Liu, Song, Ma: "Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir." in: International journal of molecular sciences, Vol. 17, Issue 6, 2017

Hu, Du, Li, Gao, Chen, Yu: "Inhibition of cerebral ischemia/reperfusion injury-induced apoptosis: nicotiflorin and JAK2/STAT3 pathway." in: Neural regeneration research, Vol. 12, Issue 1, pp. 96-102, 2017

Xu, Tan, Jiang, Chen, Lai, Zhang, Liang: "Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure." in: Chinese medicine, Vol. 12, pp. 10, 2017

Zhou, Hong, Yu, Nie, Gong, Xiong, Xie: "Exopolysaccharides from Lactobacillus plantarum NCU116 induce c-Jun dependent Fas/Fasl-mediated apoptosis via TLR2 in mouse intestinal epithelial cancer cells." in: Scientific reports, Vol. 7, Issue 1, pp. 14247, 2017

Fu, Liu, Li, Chen, Yao: "Antioxidative and Antiapoptotic Effects of Delta-Opioid Peptide [D-Ala2, D-Leu5] Enkephalin on Spinal Cord Ischemia-Reperfusion Injury in Rabbits." in: Frontiers in neuroscience, Vol. 11, pp. 603, 2017

Zheng, Chen, Zhu, Li, Deng, Wang, Zhang, Chen: "Liraglutide prevents fast weight gain and β-cell dysfunction in male catch-up growth rats." in: Experimental biology and medicine (Maywood, N.J.), Vol. 240, Issue 9, pp. 1165-76, 2016

Han, Liu, Li, Tian, Han, Wang, Fu, Guo, Zhu, Du, Tian: "HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142387, 2016

Ji, Geng, Zhou, Wei, Chen: "Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells." in: Neural regeneration research, Vol. 11, Issue 12, pp. 1962-1968, 2016

Kan, Zhou, Jin, Yang: "Effects of PDTC on NF-?B expression and apoptosis in rats with severe acute pancreatitis-associated lung injury." in: International journal of clinical and experimental medicine, Vol. 8, Issue 3, pp. 3258-70, 2015

Xu, Liu, Zhang, Wang, Wang, Yang, Huo, Sun: "Apoptosis-related protein expression in rabbits with blast brain injury following early hyperbaric oxygen therapy." in: Neural regeneration research, Vol. 7, Issue 17, pp. 1318-24, 2015

Images

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details Handling References for anti-Caspase 3 antibody (ABIN3043802) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 67-175) antibody (ABIN3043802) Anti-Caspase3 antibody, IHC(P) IHC(P): Mouse Intestine Tissue
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 67-175) antibody (ABIN3043802) Anti- Caspase3 antibody, Western blotting All lanes: Anti Caspase3 at 0.5ug/ml WB: R...
Immunohistochemistry (IHC) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 67-175) antibody (ABIN3043802) Anti-Caspase3 antibody, IHC(P) IHC(P): Rat Intestine Tissue
Immunohistochemistry (IHC) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 67-175) antibody (ABIN3043802) Anti-Caspase3 antibody, IHC(P) IHC(P): Human Lung Cancer Tissue
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 67-175) antibody (ABIN3043802) Anti- Caspase3 antibody, Western blotting All lanes: Anti Caspase3 at 0.5ug/ml Lane ...
Flow Cytometry (FACS) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 67-175) antibody (ABIN3043802) Flow Cytometry analysis of K562 cells using anti-CASP3 antibody . Overlay histogram s...
Flow Cytometry (FACS) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 67-175) antibody (ABIN3043802) Flow Cytometry analysis of K562 cells using anti-CASP3 antibody . Overlay histogram s...