anti-Rat (Rattus) Caspase 4 antibody for Immunohistochemistry

Recommended Caspase 4 Antibody (supplied by: Log in to see )

Antigen
Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) Antibodies
  • ICE(rel)II
  • ICEREL-II
  • ICH-2
  • Mih1/TX
  • TX
  • Casp11
  • CASP13
  • CASP1
  • CASP4
  • Caspl
  • ich-3
  • Casp4
  • caspase 4
  • caspase 4, apoptosis-related cysteine peptidase
  • caspase-4
  • CASP4
  • Casp4
  • LOC100731032
Reactivity
Human, Mouse (Murine), Rat (Rattus)
206
118
57
22
3
1
1
Host
Rabbit
216
28
21
17
7
Clonality
Polyclonal
Conjugate
This Caspase 4 antibody is un-conjugated
19
16
5
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
266
96
95
59
31
25
19
18
17
13
10
3
2
2
1
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Catalog No. ABIN6260482
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.59322 ABIN1031750 IHC ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN4288060 ELISA IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN3183668 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN1453020 IHC ELISA WB Rabbit IgG AA 75-124 Log in to see Polyclonal 0
1 ABIN6260486 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1002002 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN2893038 IHC IHC (p) WB Rabbit Glu124 Log in to see Polyclonal 0
1 ABIN2617985 IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN2885852 IHC ELISA WB Rabbit IgG AA 75-124 Log in to see Polyclonal 0
1 ABIN6104823 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN1886902 IHC ELISA WB Rabbit AA 91-105 Log in to see Polyclonal 0
1 ABIN2858350 IHC WB Rabbit Log in to see Polyclonal 0

General

Antigen Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(206), (118), (57), (22), (3), (1), (1)
Host Rabbit
(216), (28), (21), (17), (7)
Clonality
Polyclonal
Conjugate This Caspase 4 antibody is un-conjugated
(19), (16), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(266), (96), (95), (59), (31), (25), (19), (18), (17), (13), (10), (3), (2), (2), (1)
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Product Details anti-Caspase 4 Antibody

Target Details Caspase 4 Application Details Handling Images
Specificity Caspase 4 Antibody detects endogenous levels of total Caspase 4
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide derived from human Caspase 4
Isotype IgG
Plasmids, Primers & others

Target Details Caspase 4

Product Details anti-Caspase 4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Caspase 4 (CASP4 Antibody Abstract)
Background

Description: Inflammatory caspase (PubMed:7797510, PubMed:23516580, PubMed:25119034). Essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS (PubMed:22246630, PubMed:26174085, PubMed:26173988, PubMed:26508369, PubMed:25964352). Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed:24879791, PubMed:25964352). Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection (PubMed:25121752). In later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion, this activity may not require caspase enzymatic activity (PubMed:26508369). Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer's patient brains (PubMed:15123740, PubMed:22246630, PubMed:23661706). Activated by direct binding to LPS without the need of an upstream sensor (PubMed:25119034). Does not directly process IL1B (PubMed:7743998, PubMed:7797592, PubMed:7797510). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590).

Gene: CASP4

Molecular Weight 43 kDa
Gene ID 837
UniProt P49662
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-Caspase 4 Antibody Target Details Caspase 4 Handling Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200
Restrictions For Research Use only

Handling

Product Details anti-Caspase 4 Antibody Target Details Caspase 4 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-Caspase 4 Antibody Target Details Caspase 4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) antibody (ABIN6260482) Western blot analysis of Caspase 4 expression in mouse brain and lung.
Immunohistochemistry (IHC) image for anti-Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) antibody (ABIN6260482) ABIN6268676 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyd...
Immunohistochemistry (IHC) image for anti-Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) antibody (ABIN6260482) Colon tissue Caspase 4 antibody used at 1/200 on formalin-fixed paraffin embedded tis...