anti-Human Caspase 8 antibody for Immunoprecipitation

Recommended Caspase 8 Antibody (supplied by: Log in to see )

Antigen
Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies
  • casp8-A
  • xCaspase-8
  • casp8
  • CASP8
  • CASP-8
  • FLICE
  • MACH
  • Mch5
  • ALPS2B
  • CAP4
  • Casp-8
  • MCH5
  • caspase-8
  • zgc:92075
  • caspase 8 L homeolog
  • death related ced-3/Nedd2-like protein
  • caspase 8
  • caspase-8
  • caspase-8-like
  • caspase 8, apoptosis-related cysteine peptidase
  • casp8.L
  • LOC661095
  • CASP8
  • LOC5563818
  • casp8
  • LOC100222284
  • Casp8
Epitope
AA 181-478
40
15
14
12
11
11
11
9
7
7
7
7
6
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
345
135
105
21
21
3
1
Host
Mouse
302
83
15
2
Clonality
Monoclonal
Conjugate
This Caspase 8 antibody is un-conjugated
20
14
7
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
369
124
111
52
52
33
31
26
20
13
13
8
7
5
2
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Catalog No. ABIN1168996
$ 586.19
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19 ABIN6000174 IF/ICC IHC IP WB Rabbit AA 7-216 Log in to see Polyclonal 0
15.874249 ABIN252118 ICC IHC (fro) IF IHC (p) IP IHC WB Rabbit Large Subunit, Small Subunit, active Log in to see Polyclonal 43
13.280443 ABIN1168997 ICC IP WB Mouse IgG2b AA 181-478 Log in to see C15 0
13.280443 ABIN2135489 IP WB Mouse IgG1 N-Term Log in to see C502S 0
13.280443 ABIN5617722 IP WB Mouse IgG1 Log in to see C502S 0
13.280443 ABIN1690183 IP Magnetic Particles Rabbit Log in to see Polyclonal 0
13.280443 ABIN1690531 IP Sepharose Rabbit Log in to see Polyclonal 0
13 ABIN2899507 IF/ICC IHC IP WB Rabbit AA 217-384 Log in to see Polyclonal 0
13 ABIN2899508 IF/ICC IHC IP WB Rabbit AA 385-479 Log in to see Polyclonal 0
8.5 ABIN537399 IHC (fro) IHC (p) IP Rabbit Log in to see Polyclonal 2
7 ABIN957191 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 0
5.5 ABIN223019 IP WB Mouse IgG1 N-Term, active Log in to see C502S 5
4 ABIN957192 IHC IHC (fro) IHC (p) IP WB Rabbit AA 449-464, AA 464-479, AA 481-496, AA 523-538 Log in to see Polyclonal 0
4 ABIN1732676 IHC (p) IP WB Rabbit IgG full length Log in to see Polyclonal 0
4 ABIN537400 IHC (fro) IHC (p) IP Rabbit Log in to see Polyclonal 0
1 ABIN5688366 ICC IP WB Mouse IgG2b AA 181-478 Log in to see C15 0
1 ABIN2962132 ICC IP WB Mouse IgG2b AA 181-478 Log in to see C15 0
1 ABIN1977287 ICC IP WB Mouse IgG2b AA 181-478 Log in to see 0
1 ABIN219263 IP ELISA WB Mouse IgG1 C-Term Log in to see 0
1 ABIN2219275 IP IHC WB Rabbit IgG C-Term, active Log in to see Polyclonal 0

General

Antigen Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies
Epitope AA 181-478
(40), (15), (14), (12), (11), (11), (11), (9), (7), (7), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(345), (135), (105), (21), (21), (3), (1)
Host Mouse
(302), (83), (15), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 8 antibody is un-conjugated
(20), (14), (7), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
(369), (124), (111), (52), (52), (33), (31), (26), (20), (13), (13), (8), (7), (5), (2), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-Caspase 8 Antibody

Target Details Caspase 8 Application Details Handling References for anti-Caspase 8 antibody (ABIN1168996) Images
Specificity Recognizes the p18 subunit of human caspase-8.
Cross-Reactivity Human
Purification Purified from concentrated hybridoma tissue culture supernatant.
Purity >95 % (SDS-PAGE)
Immunogen Recombinant human caspase-8 (aa 181-478).
Clone C15
Isotype IgG2b
Plasmids, Primers & others

Target Details Caspase 8

Product Details anti-Caspase 8 Antibody Application Details Handling References for anti-Caspase 8 antibody (ABIN1168996) Images back to top
Antigen
Alternative Name Caspase-8 (CASP8 Antibody Abstract)
Background Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p18, respectively, which leads to the generation of the active caspase-8 heterotetramer (p18/p10)2.
UniProt Q14790
Pathways Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades

Application Details

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Handling References for anti-Caspase 8 antibody (ABIN1168996) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details References for anti-Caspase 8 antibody (ABIN1168996) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer In PBS containing 10 % glycerol and 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date 12 months

References for anti-Caspase 8 antibody (ABIN1168996)

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling Images back to top
Product cited in:

Leverkus, Sprick, Wachter, Mengling, Baumann, Serfling, Bröcker, Goebeler, Neumann, Walczak: "Proteasome inhibition results in TRAIL sensitization of primary keratinocytes by removing the resistance-mediating block of effector caspase maturation." in: Molecular and cellular biology, Vol. 23, Issue 3, pp. 777-90, 2003

Sprick, Rieser, Stahl, Grosse-Wilde, Weigand, Walczak: "Caspase-10 is recruited to and activated at the native TRAIL and CD95 death-inducing signalling complexes in a FADD-dependent manner but can not functionally substitute caspase-8." in: The EMBO journal, Vol. 21, Issue 17, pp. 4520-30, 2002

Létuvé, Druilhe, Grandsaigne, Aubier, Pretolani: "Involvement of caspases and of mitochondria in Fas ligation-induced eosinophil apoptosis: modulation by interleukin-5 and interferon-gamma." in: Journal of leukocyte biology, Vol. 70, Issue 5, pp. 767-75, 2001

Sprick, Weigand, Rieser, Rauch, Juo, Blenis, Krammer, Walczak: "FADD/MORT1 and caspase-8 are recruited to TRAIL receptors 1 and 2 and are essential for apoptosis mediated by TRAIL receptor 2." in: Immunity, Vol. 12, Issue 6, pp. 599-609, 2000

Cuvillier, Mayhew, Janoff, Spiegel: "Liposomal ET-18-OCH(3) induces cytochrome c-mediated apoptosis independently of CD95 (APO-1/Fas) signaling." in: Blood, Vol. 94, Issue 10, pp. 3583-92, 1999

Medema, Scaffidi, Krammer, Peter: "Bcl-xL acts downstream of caspase-8 activation by the CD95 death-inducing signaling complex." in: The Journal of biological chemistry, Vol. 273, Issue 6, pp. 3388-93, 1998

Scaffidi, Fulda, Srinivasan, Friesen, Li, Tomaselli, Debatin, Krammer, Peter: "Two CD95 (APO-1/Fas) signaling pathways." in: The EMBO journal, Vol. 17, Issue 6, pp. 1675-87, 1998

Scaffidi, Medema, Krammer, Peter: "FLICE is predominantly expressed as two functionally active isoforms, caspase-8/a and caspase-8/b." in: The Journal of biological chemistry, Vol. 272, Issue 43, pp. 26953-8, 1997